สืบค้นงานวิจัย
Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv
Danpaiboon W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv
ผู้แต่ง / หัวหน้าโครงการ (EN): Danpaiboon W.
บทคัดย่อ (EN): Venomous snakebites are an important health problem in tropical and subtropical countries. King cobra (Ophiophagus hannah) is the largest venomous snake found in South and Southeast Asia. In this study, the O. hannah venom proteome and thevenom components cross-reactive to N. kaouthia monospecific antivenin were studied. O. hannah venom consisted of 14 different protein families, including three finger toxins, phospholipases, cysteine-rich secretory proteins, cobra venom factor, muscarinic toxin, L-amino acid oxidase, hypothetical proteins, low cysteine protein, phosphodiesterase, proteases, vespryn toxin, Kunitz, growth factor activators and others (coagulation factor, endonuclease, 5'-nucleotidase). N. kaouthia antivenin recognized several functionally different O. hannah venom proteins and mediated paratherapeutic efficacy by rescuing the O. hannah envenomed mice from lethality. An engineered human ScFv specific to N. kaouthia long neurotoxin (NkLN-HuScFv) cross-neutralized the O. hannah venom and extricated the O. hannah envenomed mice from death in a dose escalation manner. Homology modeling and molecular docking revealed that NkLN-HuScFv interacted with residues in loops 2 and 3 of the neurotoxins of both snake species, which are important for neuronal acetylcholine receptor binding. The data of this study are useful for snakebite treatment when and where the polyspecific antivenin is not available. Because the supply of horse-derived antivenin is limited and the preparation may cause some adverse effects in recipients, a cocktail of recombinant human ScFvs for various toxic venom components shared by different venomous snakes, exemplified by the in vitro produced NkLN-HuScFv in this study, should contribute to a possible future route for an improved alternative to the antivenins. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901236392&doi=10.3390%2ftoxins6051526&partnerID=40&md5=315b8f9fc8e254a6eee537da645e057c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tandem Mass Spectrometry
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Danpaiboon W.. "Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv". ไม่ระบุหน่วยงาน. ม.ป.ป.

Danpaiboon W.. "Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv
Danpaiboon W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Human monoclonal scfvs that neutralize fribrinogenolytic activity of kaouthiagin, a zinc-metalloproteinase in cobra (Naja kaouthia) venom ScFv Antibody Specific to Cassiicolin, a Host-Selective Toxin of Corynespora cassiicola Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp In vitro neutralization of yellow head virus infection in shrimp using recombinant PmYRP65 protein The development of the cloaca in the human embryo Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Dihydrofolate-reductase mutations in plasmodium knowlesi appear unrelated to selective drug pressure from putative human-to-human transmission in Sabah, Malaysia Microparticle and anti-influenza activity in human respiratory secretion Comparative proteomic study of dog and human saliva Probing the origins of human acetylcholinesterase inhibition via QSAR modeling and molecular docking
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair