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Development and mapping of functional expressed sequence tag-derived simple sequence repeat markers in a rubber tree RRIM600 × PB217 population
Nirapathpongporn K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development and mapping of functional expressed sequence tag-derived simple sequence repeat markers in a rubber tree RRIM600 × PB217 population
ผู้แต่ง / หัวหน้าโครงการ (EN): Nirapathpongporn K.
บทคัดย่อ (EN): Sets of polymorphic expressed sequence tag–simple sequence repeat (EST-SSR) markers from the rubber tree (Hevea brasiliensis) have been published by many researchers, but none has been specifically developed to study latex and wood yield traits. In this study, a total 10,321 rubber tree EST sequences, generated from suppression subtractive hybridization-cDNA libraries of bark and latex of high- and low-yielding clones, were used as sources for SSR searching. A total of 432 EST-SSR loci were identified and it was possible to design primer pairs for a subset of 298 EST-SSRs. The highest proportion of EST-SSRs was represented by dinucleotide repeats (46.6 %), followed by trinucleotide repeats (44.3 %). Based on BLASTX analysis, 234 ESTs (80 %) showed similarity to genes in NCBI databases and could be divided into 120 putative proteins with known function and 114 unknown proteins. To enhance the resolution of an existing linkage map from previous work on a rubber tree RRIM600 × PB217 population, 69 EST-SSR markers from the above set were tested to be integrated into the reference genetic map. The enriched map of 18 linkage groups spanned 2054.2 cM in length, showed an average genetic distance of 4.3 cM between adjacent markers, and included 63 new EST-SSR markers. The enhanced map from this study provides a basis for comparative mapping using PCR-based markers and identification of expressed genes possibly affecting important traits of interest. © 2016, Springer Science+Business Media Dordrecht.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961763346&doi=10.1007%2fs11032-016-0461-3&partnerID=40&md5=ab803fe664a961a58148e980d0047f07
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Rubber tree
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Development and mapping of functional expressed sequence tag-derived simple sequence repeat markers in a rubber tree RRIM600 × PB217 population
Nirapathpongporn K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Identification of Rubber Clones (Hevea brasiliensis) Using Inter Simple Sequence Repeat (ISSR) Markers Genetic Linkage Map of Cassava (Manihot esculenta Crantz) Based on Rubber Tree and Cassava Simple Sequence Repeat Markers Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. Components of Inorganic Phosphates in Rhizosphere of Rubber Tree (Hevea brasiliensis) Latex Harvesting Technologies Adapted to Clones IRCA18, IRCA 111, IRCA 130, PB 235 and PB 260 of Hevea brasiliensis (Rubber Tree) of the Class to Active Metabolism in South-Western Côte d’Ivoire Chloroplast DNA Microsatellite Differentiation in Cultivated and Wild Rubber Tree (Hevea brasiliensis) Development and Promotion of Rubber (Hevea brasiliensis Muell Arg.) Towards Commercialization in Oriental and Occidental Mindoro Transcriptome Analyses Reveal Molecular Mechanism Underlying Tapping Panel Dryness of Rubber Tree (Hevea brasiliensis) Molecular characterization and genetic relationship of marigolds (Tagetes spp.) based on simple sequence repeat markers การพัฒนาแผนที่พันธุกรรมยางพาราของพันธุ์ RRIM 600 x PB 217 โดยการใช้ microsatellite markers
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