คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Siriphan Saeng-aroon

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand. 2 Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand. 3 Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Thailand. 4 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand. 5 Research Institute of Microbial Diseases, Osaka University, Suita, Japan. 6 Osaka Institute of Public Health, Osaka, Japan.

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Use of dried blood spot specimens for in-house HIV drug resistance genotyping assay among infants in Thailand Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene National Survey of Pretreatment HIV-Drug Resistance in Thai HIV-1-Infected Adults Mangrove forests in Thailand Progress on Rubber Breeding of Thailand

คัดลอก URL

ชื่อเรื่อง (EN):	External Quality Assessment Scheme for HIV-1 Drug Resistance Genotyping in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Siriphan Saeng-aroon
บทคัดย่อ (EN):	The efficacy of antiretroviral therapy (ARV) can be compromised by the emergence and transmission of HIV-1 drug-resistant strains. HIV-1 drug resistance (DR) genotyping testing thus plays an important role in the selection of optimal treatment regimens for HIV infected individuals. Given the complexities of the testing procedures and the variety of approaches used, there is considerable potential for results to vary between laboratories. In Thailand, the national External Quality Assessment (EQA) scheme assesses the DR genotype testing performance of laboratories. Here we evaluated the performance of laboratories in nucleotide sequencing and compared drug resistance-associated mutations (DRMs) in the HIV-1 protease (PR) and reverse transcriptase (RT) genes during 2010–2015. The EQA samples in the 12 panels showed predominance for the CRF01_AE (85%) and subtype B (15%). Fourteen laboratory datasets were generated: eight using TruGene (TG), two using ViroSeq (VS), and four using in-house (IH) assays. All IH and VS laboratories had penalty scores <7, whereas five of the eight TG laboratories had fluctuating penalty scores. Moreover, seven and six TG laboratories could not amplify the two identical samples, 10B and 10E samples, or the CRF01_AE. This result raised the concern that current primers in the TG kit might have some mismatches with CRF01_AE. Our findings demonstrate the requirement for laboratory participation in the ongoing EQA program and the optimization of kit assays using CRF01_AE samples. Our results may also indicate that one advantage of participation is that the laboratories can monitor and investigate the source of lab errors.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=538
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand. 2 Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand. 3 Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Thailand. 4 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand. 5 Research Institute of Microbial Diseases, Osaka University, Suita, Japan. 6 Osaka Institute of Public Health, Osaka, Japan.
คำสำคัญ (EN):	EQA; HIV; Drug resistance genotyping testing; CRF01_AE
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science