สืบค้นงานวิจัย
Immune response to recombinant Burkholderia pseudomallei FliC
Koosakulnirand S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Immune response to recombinant Burkholderia pseudomallei FliC
ผู้แต่ง / หัวหน้าโครงการ (EN): Koosakulnirand S.
บทคัดย่อ (EN): Burkholderia pseudomallei is a flagellated Gram-negative bacterium which is the causative agent of melioidosis. The disease poses a major public health problem in tropical regions and diabetes is a major risk factor. The high mortality rate of melioidosis is associated with severe sepsis which involves the overwhelming production of pro-inflammatory cytokines. Bacterial flagellar protein (flagellin) activates Toll-like receptor 5 (TLR5)-mediated innate immune signaling pathways and induces adaptive immune response. However, previous studies of TLR5 signaling in melioidosis have been performed using recombinant flagellin from Salmonella Typhimurium instead of B. pseudomallei. This study aimed to investigate human innate immune response and antibody response against a recombinant B. pseudomallei flagellin (rFliC). We prepared B. pseudomallei rFliC and used it to stimulate HEK-Blue TM -hTLR5 and THP1-Dual TM cells to assess TLR5 activation. Subsequently, whole blood stimulation assays with rFliC were performed ex vivo. TLR5-flagellin interactions trigger activation of transcription factor NF-κB in HEK-Blue TM -hTLR5 cells. Pro-inflammatory cytokine (IL-1β, IL-6, and TNF-α) productions from whole blood in response to rFliC differed between fourteen healthy individuals. The levels of these cytokines changed in a dose and time-dependent manner. ELISA was used to determine rFliC-specific antibodies in serum samples from different groups of melioidosis patients and healthy subjects. IgG antibody to rFliC in melioidosis patients with diabetes were higher compared with non-diabetic patients. Our results show that B. pseudomallei flagellin is a potent immune stimulator and that the immune responses to rFliC are different among individuals. This may provide valuable insights toward the potential use of rFliC in vaccine development. © 2018 Koosakulnirand et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056605964&doi=10.1371%2fjournal.pone.0198906&partnerID=40&md5=01f9855752376c9c99b605e687995986
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Recombinant Proteins
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Koosakulnirand S.. "Immune response to recombinant Burkholderia pseudomallei FliC". ไม่ระบุหน่วยงาน. ม.ป.ป.

Koosakulnirand S.. "Immune response to recombinant Burkholderia pseudomallei FliC". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Immune response to recombinant Burkholderia pseudomallei FliC
Koosakulnirand S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Soil nutrient depletion is associated with the presence of Burkholderia pseudomallei Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia specie The appearance of allelopathic substances affecting the presence of Burkholderia pseudomallei Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei Insights into the naturally acquired immune response to Plasmodium vivax malaria The effects of feeding immunostimulant β-glucan on the immune response of Pangasianodon hypophthalmus Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei การเฝ้าระวังเชื้อ Burkholderia pseudomallei ในสัตว์จากโรงฆ่าสัตว์ในจังหวัดนครปฐมและราชบุรี Utilization of whole-cell MALDI-TOF mass spectrometry to differentiate Burkholderia pseudomallei wild-type and constructed mutants Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models o
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