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Antimicrobial susceptibility and toxin production of Clostridium difficile isolated from diarrheal patients during 2012-2015
Piyada Wangroongsarb - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Antimicrobial susceptibility and toxin production of Clostridium difficile isolated from diarrheal patients during 2012-2015
ผู้แต่ง / หัวหน้าโครงการ (EN): Piyada Wangroongsarb
บทคัดย่อ (EN): Background: Clostridium difficile infection affects people who have been treated with antibiotics and long-term care facilities. C. difficile release two protein toxins, toxin A and toxin B that are the major virulence factors. C. difficile plays important role in hospital-associated diarrhea around the world with wide range of clinical symptoms from asymptomatic carrier, mild to severe diarrhea, colitis, pseudomembranous colitis. Objectives: To investigate the prevalence of toxigenic C. difficile by multiplex PCR and to determine antimicrobial susceptibility of C. difficile isolated from diarrheal patients using agar dilution method. Materials and methods: A total of 49 isolates of C. difficile from stool specimens of diarrheal patients with suspected antibiotic-associated diarrhea during 2012-2015 were included. Detection of toxin genes by multiplex PCR and minimum inhibitory concentration (MIC) by agar dilution were performed. Results: C. difficile isolates were classified into three groups according to toxin genes found as following 1) negative detection of types A and B toxin genes (A-B-) (29 isolates, 3.36%), 2) negative detection of type A toxin gene but positive detection of type B toxin gene (A-B+) (14 isolates, 1.62%), 3) positive detection of type A and B toxin genes (A+B+) (6 isolates, 0.69%). Binary toxin genes were not found in any isolate. Results of MIC for 9 drugs were as following; all isolates were sensitive to vancomycin (MIC 0.25-1 µg/mL) and Metronidazole (MIC 0.25-2 µg/mL). All isolates were resistant to clindamycin (MIC=8 to >256 µg/ml, MIC50=32 µg/mL). Approximately 65.7% of isolates were sensitive to moxifloxacin (MIC 1-4 µg/mL). Most isolates were sensitive to tetracycline (MIC128 µg/mL (MIC50=4 µg/mL), 0.5-32 µg/mL (MIC50=1 µg/mL) and 0.125-128 µg/mL (MIC50=2 µg/mL), respectively. Conclusion: Antimicrobial susceptibility and toxin production of C. difficile are useful for monitoring the severity of disease and rate of resistant to antibiotics. The study found that the majority of metronidazole and vancomycin were effective for treatment of C. difficile diarrheal infection in Thailand
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=510
เผยแพร่โดย (EN): Department of Medical Sciences, National Institute of Health, Nonthaburi Province, Thailand
คำสำคัญ (EN): Clostridium difficile; agar dilution; minimum inhibitory concentration (MIC); antimicrobial susceptibility
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Antimicrobial susceptibility and toxin production of Clostridium difficile isolated from diarrheal patients during 2012-2015
Piyada Wangroongsarb
Department of Medical Sciences, National Institute of Health, Nonthaburi Province, Thailand
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