คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Nitchakarn Noranate

เผยแพร่โดย

1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand 2 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan 3 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand

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งานวิจัยที่แนะนำ Characterization of chikungunya virus-like particles ความชุกของแอนติบอดีต่อ Coxsackie B Virus ในตัวอย่างผู้ป่วยในประเทศไทยระหว่างปี พ.ศ. 2557-2561 Prevalence of Coxsackie B Virus antibodies inpatient serum specimens in Thailand during 2014-2018 The use of green fluorescent protein-tagged virus-like particles as a tracer in the early phase of chikungunya infection Anti-Chikungunya Virus Monoclonal Antibody That Inhibits Viral Fusion and Release Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus Characterization of an unusual DS-1-like G8P[8] rotavirus strain from Japan in 2017: evolution of emerging DS-1-like G8P[8] strains through reassortment Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus ICP35 is a TREX-like protein identified in white spot syndrome virus

คัดลอก URL

ชื่อเรื่อง (EN):	Characterization of Chikungunya Virus-Like Particles
ผู้แต่ง / หัวหน้าโครงการ (EN):	Nitchakarn Noranate
บทคัดย่อ (EN):	Chikungunya virus (CHIKV) is becoming a global concern due to the increasing number of outbreaks throughout the world and the absence of any CHIKV-specific vaccine or treatment. Virus-like particles (VLPs) are multistructured proteins that mimic the organization and conformation of native viruses but lack the viral genome. They are noninfectious and potentially safer vaccine candidates. Recent studies demonstrated that the yield of CHIKV VLPs varies depending on the strains, despite the 95% amino acid similarity of the strains. This might be due to the codon usage, since protein expression is differently controlled by different organisms. We optimized the region encoding CHIKV structural proteins, C-E3-E2-6k-E1, inserted it into a mammalian expression vector, and used the resulting construct to transfect 293 cells. We detected 50-kDa proteins corresponding to E1 and/or E2 in the cell lysate and the supernatant. Transmission electron microscopy revealed spherical particles with a 50- to 60-nm diameter in the supernatant that resembled the native CHIKV virions. The buoyant density of the VLPs was 1.23 g/mL, and the yield was 20 µg purified VLPs per 108 cells. The VLPs aggregated when mixed with convalescent sera from chikungunya patients, indicating that their antigenicity is similar to that of native CHIKV. Antibodies elicited with the VLPs were capable of detecting native CHIKV, demonstrating that the VLPs retain immunogenicity similar to that of the native virion. These results indicated that CHIKV VLPs are morphologically, antigenically, and immunologically similar to the native CHIKV, suggesting that they have potential for use in chikungunya vaccines.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=454
เผยแพร่โดย (EN):	1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand 2 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan 3 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand
คำสำคัญ (EN):	Chikungunya Virus-Like Particles
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science