สืบค้นงานวิจัย
Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon
Ngernsoungnern P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon
ผู้แต่ง / หัวหน้าโครงการ (EN): Ngernsoungnern P.
บทคัดย่อ (EN): In penaeoid shrimp, contact of spawned eggs with seawater induces egg activation. However, little is known about the factors that influence egg activation in Penaeus monodon. Therefore, the main objective of the present study was to determine whether shrimp-produced proteases that are released in seawater are essential for egg activation. Female shrimp were allowed to spawn in artificial seawater containing protease inhibitors. It was shown that 4-amidinophenylmethanesulfonyl fluoride hydrochloride (APMSF) and soybean trypsin inhibitor (SBTI) inhibited egg activation. High doses of APMSF and SBTI induced only 1–2% complete egg activation. Moreover, when the APMSF- and SBTI- treated eggs were subsequently washed, egg activation did not resume. In contrast, other protease inhibitors, pepstatin A, E-64, and ethylene glycol tetraacetic acid, did not inhibit egg activation, as evident by approximately 98% complete activation. Our results suggest that serine proteases, which are most likely trypsin-like proteases, released in seawater may be involved in egg activation of P. monodon. © 2015 Taylor & Francis.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84945242353&doi=10.1080%2f07924259.2015.1086827&partnerID=40&md5=1d0d00af871b59f0fe3e377275c3df0a
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Protease inhibitor
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon
Ngernsoungnern P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection A novel localization of molt-inhibiting hormone in the tegumental glands of shrimp Penaeus monodon and its possible role in shrimp molting A novel function of bursicon in stimulation of vitellogenin expression in black tiger shrimp, Penaeus monodon An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon Optimisation of electroporation and lipofection protocols to derive the black tiger shrimp cell line (Penaeus monodon) A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp (ภาษาอังกฤษ) Functional characterization of a novel C-type lectin gene in the black tiger shrimp, Penaeus monodon Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon Effects of fatty acids enrichment on polychaetes to be used as a maturation feed for the male black tiger shrimp (Penaeus monodon)
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