สืบค้นงานวิจัย
Detection of Leishmania DNA in saliva among patients with HIV/AIDS in Trang Province, southern Thailand
Pandey N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Detection of Leishmania DNA in saliva among patients with HIV/AIDS in Trang Province, southern Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Pandey N.
บทคัดย่อ (EN): Leishmaniasis is a neglected tropical disease causing opportunistic infection among patients with HIV/AIDS. The fatal form of this disease is visceral leishmaniasis (VL). DNA of Leishmania can be detected in saliva, for which the collection is noninvasive and requires little expertise. This study aimed to evaluate the sensitivity and specificity of a nested-PCR to amplify the Internal Transcribed Spacer 1 (ITS1) to detect Leishmania DNA in paired saliva and buffy coat samples of 305 Thai patients with HIV/AIDS in Trang Hospital, Trang Province, southern Thailand. For asymptomatic Leishmania infection among Thai patients with HIV/AIDS, the sensitivity and specificity of the nested-PCR-ITS1 in buffy coat were 73.9 and 100%, respectively. However, the sensitivity in saliva was 26.1% and specificity was 100%. Using the nested-PCR-ITS1, saliva and buffy coat samples showed positive agreement in only 52.0% of patients. Saliva tested results with the nested-PCR-ITS1 showed positive agreement with the Direct Agglutination Test (DAT) in 46.5% of patients. Only 12.1% of the samples showed positive agreement for Leishmania infection among all the three tests: saliva, buffy coat and DAT results. Using nucleotide sequencing, at least three species of Leishmania infection were identified in saliva, i.e., L. siamensis (n = 28), L. martiniquensis (n = 9), and L. donovani complex (n = 1). As a result, buffy coat still appears to be a better specimen to diagnose asymptomatic VL infection among individuals with HIV. However, the use of both buffy coat and saliva together as clinical specimens would increase the sensitivity of Leishmania detection. © 2018 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048422362&doi=10.1016%2fj.actatropica.2018.06.006&partnerID=40&md5=55d3a91fcdde329d40be20e8b98f04ee
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thailand
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Pandey N.. "Detection of Leishmania DNA in saliva among patients with HIV/AIDS in Trang Province, southern Thailand". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Detection of Leishmania DNA in saliva among patients with HIV/AIDS in Trang Province, southern Thailand
Pandey N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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