คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Benjawan Phetsuksiri

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand 2 Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Japan 3 Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Japan 4 Section of Bacterial Infections, Research Collaboration Center for Emerging and Re-emerging Infectious Diseases, Thailand 5 Research Institute for Microbial Diseases, Osaka University, Japan

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คัดลอก URL

ชื่อเรื่อง (EN):	Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis
ผู้แต่ง / หัวหน้าโครงการ (EN):	Benjawan Phetsuksiri
บทคัดย่อ (EN):	The diagnosis of tuberculosis (TB) in endemic countries is challenging due to high caseloads and limited resources. A simple and cost-effective diagnostic test for the rapid detection of Mycobacterium tuberculosis (M. tuberculosis) in clinical specimens is crucially needed. We evaluated the performance of an in-house assay based on loop-mediated isothermal amplification (LAMP) targeting the M. tuberculosis 16S ribosomal RNA (rRNA) gene for the diagnosis of TB in Thailand. A total of 252 sputum samples from suspected cases of pulmonary TB were analyzed. The sensitivity of LAMP was 99.04% (103/104; 95% confidence interval [CI]: 94.76-9.98%) and 72.73% (16/22; 95% CI: 49.78-89.27%) for smear-positive and smear-negative samples with TB-culture positivity, respectively. LAMP detected 20.69% (24/116) of TB culture negative samples but all those were positive by conventional polymerase chain reaction (PCR). The sensitivity of LAMP was higher than that of sputum microscopy while the performance of LAMP was similar to PCR. None of the samples positive for non-tuberculous mycobacteria by culture and PCR were positive by LAMP. Compared to TB culture, the positive predictive value (PPV), negative predictive value (NPV), and kappa coefficient of LAMP were 83.22%, 88.33%, and 0.75 respectively. Based on the diagnostic performance, we propose that LAMP would be suitable as a potential diagnostic test for rapid TB diagnosis in resource-limited laboratory settings.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=577
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand 2 Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Japan 3 Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Japan 4 Section of Bacterial Infections, Research Collaboration Center for Emerging and Re-emerging Infectious Diseases, Thailand 5 Research Institute for Microbial Diseases, Osaka University, Japan
คำสำคัญ (EN):	Tuberculosis
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science