สืบค้นงานวิจัย
Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis
R.G. Kala - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): R.G. Kala
บทคัดย่อ (EN): plant regeneration system via somatic embryogenesis was developed earlier from leaf explants of Hevea brasiliensis, the major commercial source of natural rubber. In vitro culture response of Hevea leaf explants collected from plants of different physiological maturity such as seedlings, somatic plants, bud grafted plants and mature trees varied. In the present study, work was carried out for the isolation and characterization of chlorophy11 a/b binding protein gene (Cab), expressed mainly in green leaves, for which the expression is reported to be directly related to the juvenility of the plant tissues. The gene expression with respect to in vitro culture response of Hevea leaf explants collected from plants of different maturity was also studied. Cab gene was PCR amplified initially from the genomic as well as cDNA of Hevea brasiliensis using primers designed from the conserved regions of the Cab gene reported earlier from related plant species. The PCR amplified fragment (0.5 kb) WAS ELUTED, CLONED AND SEQUENCED. The sequence revealed the presence of 525 bp that showed 91% homology with Cab mRNA from Ricinus communis. Further, full length Cab gene (0.8 kb) was amplified from genomic DNA using Ricinus communis specific primers. The sequence revealed the presence of a fragment of 802 bp which showed 90% sequence homology with the reported cDNA sequence of Cqab gene from Ricinus communis. This is the first report on isolation and characterization of Cab gene from Hevea brasiliensis. Differential expression of the gene was observed in RT-PCR with cDNA synthesized from RNA isolated from source plants of different physiological maturity with a higher expression from physiologically juvenile sources. Higher expression of the gene was also observed in northern analysis in physiologically juvenile plants such as seedlings and somatic plants than in mature bud grafted clonal materials and field grown trees. Differential expression was also noted in leaves of different maturity.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): physiological maturity
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

R.G. Kala. "Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

R.G. Kala. "Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis
R.G. Kala
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis) A Mini Juvenile Bud Stick and Mini Seedling Budding of Hevea brasiliensis Functional Characterization of a Novel Hevea â-1, 3-Glucanase Gene Promoter and Its Regulatory Role in Hevea and Tobacco Tissues Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala ANALYSING THE GENETIC RESPONSE TO ENVIRONMENTAL STRESS IN YOUNG RUBBER PLANTS (HEVEA BRASILIENSIS) FOR PREDICTING ADULT PHENOTYPIC EXPRESSION Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis CLONAL VARIABILITY IN THE INCLINATION OF LATICIFERS AND PHLOIC RAYS IN HEVEA BRASILIENSIS IMPROVEMENT AND APPLICATION OF THE TECHNIQUE OF MINI-SEEDLING BUDDING OF HEVEA BRASILIENSIS CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN
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