สืบค้นงานวิจัย
Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes
Chewapreecha C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes
ผู้แต่ง / หัวหน้าโครงการ (EN): Chewapreecha C.
บทคัดย่อ (EN): Traditional genetic association studies are very difficult in bacteria, as the generally limited recombination leads to large linked haplotype blocks, confounding the identification of causative variants. Beta-lactam antibiotic resistance in Streptococcus pneumoniae arises readily as the bacteria can quickly incorporate DNA fragments encompassing variants that make the transformed strains resistant. However, the causative mutations themselves are embedded within larger recombined blocks, and previous studies have only analysed a limited number of isolates, leading to the description of “mosaic genes” as being responsible for resistance. By comparing a large number of genomes of beta-lactam susceptible and non-susceptible strains, the high frequency of recombination should break up these haplotype blocks and allow the use of genetic association approaches to identify individual causative variants. Here, we performed a genome-wide association study to identify single nucleotide polymorphisms (SNPs) and indels that could confer beta-lactam non-susceptibility using 3,085 Thai and 616 USA pneumococcal isolates as independent datasets for the variant discovery. The large sample sizes allowed us to narrow the source of beta-lactam non-susceptibility from long recombinant fragments down to much smaller loci comprised of discrete or linked SNPs. While some loci appear to be universal resistance determinants, contributing equally to non-susceptibility for at least two classes of beta-lactam antibiotics, some play a larger role in resistance to particular antibiotics. All of the identified loci have a highly non-uniform distribution in the populations. They are enriched not only in vaccine-targeted, but also non-vaccine-targeted lineages, which may raise clinical concerns. Identification of single nucleotide polymorphisms underlying resistance will be essential for future use of genome sequencing to predict antibiotic sensitivity in clinical microbiology. © 2014 Chewapreecha et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908135412&doi=10.1371%2fjournal.pgen.1004547&partnerID=40&md5=096b3f08e38d5158be40fa3915364005
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Streptococcus pneumoniae
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Chewapreecha C.. "Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chewapreecha C.. "Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes
Chewapreecha C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Single nucleotide polymorphisms in Plasmodium falciparum V type H+ pyrophosphatase gene (pfvp2) and their associations with pfcrt and pfmdr1 polymorphisms Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment ศึกษา Single Nucleotide Polymorphisms (SNPs) ในโปรโมเตอร์ของยีน accD ในปาล์มน้ำมัน Association between haplotype tagging single nucleotide polymorphisms of the ret proto-oncogene (RET) and hirschsprung disease in thais Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation Identification of Resistance of Hevea brasiliensis Clones to Corynespora Leaf Fall Disease IDENTIFICATION OF DROUGHT TOLERANT GENES BY QUANTITATIVE EXPRESSION ANALYSIS IN HEVEA BRASILIENSIS Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape Expression profiles of candidate genes associated with cyanogenic potential in cassava at different growth stages Interacting networks of resistance, virulence and core machinery genes identified by genome-wide epistasis analysis
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