สืบค้นงานวิจัย
Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus
Yoonuan T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus
ผู้แต่ง / หัวหน้าโครงการ (EN): Yoonuan T.
บทคัดย่อ (EN): Cathepsin L is a cysteine protease belonging to the papain family. In parasitic trematodes, cathepsin L plays essential roles in parasite survival and host–parasite interactions. In this study, cathepsin L of the lung fluke Paragonimus pseudoheterotremus (PpsCatL) was identified and its molecular biological and immunological features characterized. A sequence analysis of PpsCatL showed that the gene encodes a 325-amino-acid protein that is most similar to P. westermani cathepsin L. The in silico three-dimensional structure suggests that PpsCatL is a pro-enzyme that becomes active when the propeptide is cleaved. A recombinant pro-PpsCatL lacking the signal peptide (rPpsCatL), with a molecular weight of 35 kDa, was expressed in E. coli and reacted with P. pseudoheterotremus-infected rat sera. The native protein was detected in crude worm antigens and excretory–secretory products and was localized in the cecum and in the lamellae along the intestinal tract of the adult parasite. Enzymatic activity of rPpsCatL showed that the protein could cleave the fluorogenic substrate Z-Phe-Arg-AMC after autocatalysis but was inhibited with E64. The immunodiagnostic potential of the recombinant protein was evaluated with an enzyme-linked immunosorbent assay (ELISA) and suggested that rPpsCatL can detect paragonimiasis with high sensitivity and specificity (100 and 95.6 %, respectively). This supports the further development of an rPpsCatL-ELISA as an immunodiagnostic tool. © 2016, Springer-Verlag Berlin Heidelberg.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983397173&doi=10.1007%2fs00436-016-5232-x&partnerID=40&md5=f3dd1ed6ef83285f47da421364a19172
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Sequence Alignment
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Yoonuan T.. "Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus". ไม่ระบุหน่วยงาน. ม.ป.ป.

Yoonuan T.. "Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus
Yoonuan T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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