สืบค้นงานวิจัย
Anti-tumor and immune enhancing activities of rice bran gramisterol on acute myelogenous leukemia
Somintara S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Anti-tumor and immune enhancing activities of rice bran gramisterol on acute myelogenous leukemia
ผู้แต่ง / หัวหน้าโครงการ (EN): Somintara S.
บทคัดย่อ (EN): Acute myelogenous leukemia (AML) is a cancer of the blood that most commonly affects human adults. The specific cause of AML is unclear, but it induces abnormality of white blood cells that grow rapidly and accumulate in bone marrow interfering with the production and functions of the normal blood cells. AML patients face poor prognosis and low quality of life during chemotherapy or transplantation of hematopoietic stem cells due to the progressive impairment of their immune system. The goal of this study is to find natural products that have the potential to delay growth or eliminate the abnormal leukemic cells but cause less harmful effect to the body's immune system. Methods and Findings: The unsaponified fraction of Riceberry rice bran (RBDS) and the main pure compound, gramisterol, were studied for cytotoxicity and biological activities in WEHI-3 cells and in the leukemic mouse model induced by transplantation of WEHI-3 cells intraperitoneally. In the in vitro assay, RBDS and gramisterol exerted sub-G1 phase cell cycle arrest with a potent induction of apoptosis. Both of them effectively decreased cell cycle controlling proteins (cyclin D1 and cyclin E), suppressed cellular DNA synthesis and mitotic division, and reduced anti-apoptosis Bcl-2 protein, but increased apoptotic proteins (p53 and Bax) and activated caspase-3 enzyme in the intrinsic cell death stimulation pathway. In leukemic mice, daily feeding of RBDS significantly increased the amount of immune function-related cells including CD3+, CD19+, and CD11b+, and elevated the serum levels of IFN-γ, TNF-α, IL-2, and IL-12β cytokines, but suppressed IL-10 level. At the tumor sites, CD11b+ cells were polarized and became active phagocytotic cells. Treatment of mice normal immune cells with gramisterol alone or a combination of gramisterol with cytokines released from RBDS-treated leukemic mice splenocytes culture synergistically increased pSTAT1 transcriptional factor that up-regulated the genes controlling cell survival and function. Phosphorylation of STAT1 was absent in WEHI-3. Instead, similar treatments significantly decreased pSTAT3 signaling that regulates transcription of genes controlling tumor growth and proliferation. Conclusions: Rice bran gramisterol possesses a promising anti-cancer effect against a tumor of white blood cells and induces the production of anti-cancer immune-related cytokines. Gramisterol induces cell cycle arrest and apoptosis via suppression of pSTAT3 signaling control of tumor cells' growth and progression. Gramisterol increased IFN-γ production and prevented the dysfunctional immune system of leukemic mice by enhancing pSTAT1 transcription signal controlling proliferation and functions of hematopoietic cells in the spleen. Together with IFN-γ, gramisterol efficiently facilitates leukemic mice immune system modulation leading to improvement of the AML condition. Administration of RBDS containing gramisterol potentiates immune recovery of leukemic mice and extends their survival. This finding encourages the medicinal application of rice bran gramisterol as a palliative treatment or an alternative agent for future drug development against AML. Copyright © 2016 Somintara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954568005&doi=10.1371%2fjournal.pone.0146869&partnerID=40&md5=91d0767aff7b668ff6486bd92e2cf205
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tumor Suppressor Protein p53
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Somintara S.. "Anti-tumor and immune enhancing activities of rice bran gramisterol on acute myelogenous leukemia". ไม่ระบุหน่วยงาน. ม.ป.ป.

Somintara S.. "Anti-tumor and immune enhancing activities of rice bran gramisterol on acute myelogenous leukemia". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Anti-tumor and immune enhancing activities of rice bran gramisterol on acute myelogenous leukemia
Somintara S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Structures of phytosterols and triterpenoids with potential anti-cancer activity in bran of black non-glutinous rice Potential anti-inflammatory and anti-oxidative properties of Thai colored-rice extracts Anti-inflammatory and anti-insulin resistance activities of aqueous extract from Anoectochilus burmannicus Cytotoxic, anti-HIV–1 and anti-inflammatory activities of lanostanes from fruits of Garcinia speciosa Antioxidant and Anti-Inflammatory Effects of Defatted Rice Bran (Oryza Sativa L.) Protein Hydrolysates on Raw 264.7 Macrophage Cells A bioaccessible fraction of parboiled germinated brown rice exhibits a higher anti-inflammatory activity than that of brown rice Antioxidant and anti-inflammatory activities of durian and rambutan pulp extract Anti-fibrotic and anti-inflammatory effects of parboiled germinated brown rice (Oryza sativa ‘KDML 105’) in rats with induced liver fibrosis Anti-cholinesterase inhibitory activities of different varieties of chili peppers extracts The influence of extraction methods on composition and antioxidant properties of rice bran oil
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair