สืบค้นงานวิจัย
Half Ovulo Embryo Culture-An Ideal Method for Raising True-To-Type Seedlings in Hevea brasiliensis
Rekha K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Half Ovulo Embryo Culture-An Ideal Method for Raising True-To-Type Seedlings in Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Rekha K.
บทคัดย่อ (EN): Hevea brasiliensis (Pare rubber tree) is the major commercial source of natural rubber because of its abundance in the latex. High quality, and convenience of harvesting. Rubber clones are propagated by bud grafting of the desired scion on to assorted root stocks grown from cross pollinated seeds. Since the seedlings are genetically divergent, this has been often implicated as the source of large tree to tree variation in growth and yield of bud grafted Hevea trees. Dependence on bud grafting has become inevitable for the propagation of selected clones and maintenance of clonal integrity. It has been reported that there is isozyme polymorphism between different trees of the same clone, due to the variable root stocks. Uniform rootstocks will be an effective solution to reduce intra-clonal variation due to stock-scion interaction. Consequently, cloning the root system is a major challenge in Hevea breeding. Tissue culture methods provide an alternative to produce own rooted plants in Hevea. However, the major problem with this method is the failure to produce an adequate tap root necessary for tree stability. Moreover, the recalcitrance of clonal explants will not allow the production of own rooted plants to a commercial scale. In this context the present work was initiated with the objective of developing multiple, true-to-type seedlings of Hevea brasiliensis via induction of zygotic polyembryony, and to assess the uniformity/ heterogeneity among the seedlings. Immature open pollinated fruits (10-12 week’s old) of clone RRII 105 were collected from the field. Nitsch basal medium with different combinations of growth regulators viz. GA, Kinetin and Zeatin were experimented for the induction of polyembryony, following half ovulo embryo culture. Multiple embryos could be induced from a few of the cultured ovules in a combination of 2 mg/1 GA3, 3 mg/1 kinetin and 0.3 mg/1 Zeatin. In this medium, the number of embryos developed from a single ovule could be enhanced to 60 as against 12 observed n an earlier pilot study (Rekha et al 2010). Embryos recovered were transferred for maturation in the same basal medium fortified with organic supplements such as banana powder, malt extract and casein hydrolysate (100 mg/1). For germination half strength MS medium along with 0.3 mg/1 BA and 0.3 mg/1 GA was used. Multiple embryos and seedlings were developed from three independent ovules and were hardened and field planted. These plants were subjected to RAPD analysis using the standard procedure. Molecular analysis of these plants using RAPD could prove the genetic uniformity as well as zygotic origin of the multiple seedlings developed. These multiple seedlings are ideal candidates for stock-scion interaction studies for which uniform own rooted plants are so far not available. Moreover with further refinements, this method can be utilized for developing uniform rootstocks which will help to reduce intraclonal variation due to stock-scion interaction in rubber plantations.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Seedling
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Rekha K.. "Half Ovulo Embryo Culture-An Ideal Method for Raising True-To-Type Seedlings in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Rekha K.. "Half Ovulo Embryo Culture-An Ideal Method for Raising True-To-Type Seedlings in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Half Ovulo Embryo Culture-An Ideal Method for Raising True-To-Type Seedlings in Hevea brasiliensis
Rekha K.
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
A Mini Juvenile Bud Stick and Mini Seedling Budding of Hevea brasiliensis IMPROVEMENT AND APPLICATION OF THE TECHNIQUE OF MINI-SEEDLING BUDDING OF HEVEA BRASILIENSIS CLONAL VARIABILITY IN THE INCLINATION OF LATICIFERS AND PHLOIC RAYS IN HEVEA BRASILIENSIS THE YIELD AND QUALITY OF RUBBER (HEVEA BRASILIENSIS) AS AFFECTED BY IRRIGATION AND FERTILIZER APPLICATIONS Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) Seasonal Variation and Evolution of Some Latex Physiological Parameters of Hevea brasiliensis over Consecutive Tapping Years Evaluation of Half-Sib Progenies of Canopy Mutant of Hevea brasiliensis TOWARD SELECTION OF IMPROVED HEVEA BRASILIENSIS CLONES FOR SOUTH KONKAN: LONG TERM PERFORMANCE OF ORTET CLONES Effect of Nurse Culture on Inducing Division of Isolated Pollen Protoplasts of Hevea brasiliensis BIOMOLECULES CONTENTS AND RUBBER PROPERTIES OF SOME HEVEA BRASILIENSIS CLONES
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