สืบค้นงานวิจัย
การสกัดและการศึกษาคุณลักษณะของโปรตีนจากโอคารา.
ณัชชา สุพิชญางกูร. - สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหารลาดกระบัง
ชื่อเรื่อง: การสกัดและการศึกษาคุณลักษณะของโปรตีนจากโอคารา.
ชื่อเรื่อง (EN): Isolation and study on characterization of okara protein.
ผู้แต่ง / หัวหน้าโครงการ: ณัชชา สุพิชญางกูร.
บทคัดย่อ: Study on the interaction between moisture content of okara (4%, 8% and fresh >80%) and extraction time (1 and 2 hours at 38ฐC) on extracted protein yield was done. The result showed that extracted protein yield of okara which was dried at 60ฐC untill their moisture reached 4% and 8% were not different from that of fresh okara. Extracted protein yield were increased if extraction time increased from 1 hr to 2 hr The functional properties of protein would be effected by heating process (extraction and drying). Surface hydrophobicity (So) of okara protein extracted at various conditions was elucidated. So of okara protein was increased when increasing in extraction time and drying time. Protein would be partialy denatured and their structure would be unfolded during heating. The unfolding protein would expose buried hydrophobic groups to the surface of molecule. Okara protein extracted from fresh okara and used extraction time of 1 hr had So and Soulubility higher than other samples. The result found that there were significant interaction between moisture content of okara and extraction time on the emulsion activity and water holding capacity (WHC) of okara protein. WHC of okara protein extracted from dried okara was lower than those of wet okara. If the extraction time was increased from 1 to 2 hr, WHC of okara protein would be decreased. However, fat binding capacity (FBC) of okara protein would be increased if extraction time was increased from 1 to 2 hr. Moreover, foaming stability was effected by drying process. Foaming stability of okara protein that extracted from dried okara was better than that of fresh okara. Functional properties of okara protein and conventional soy protein isolate (SPI) were compared. The result showed that there was no different in funtional properties of okara protein and SPI. However, Foaming activity and FBC of okara protein were better than those of SPI. Subunit of okara protein was determined by using SDS-PAGE in the presence of 2-Mercaptoethanol. The result showed that subunit of okara protein was looked like subunits of SPI. Moreover, subunits of okara protein extracted at various experimental conditions were not different. The chemical composition of okara protein was also elucidated. The approximate analysis showed that okara protein contained 56-60% protein, 38-40% carbohydrate and 3-4% ash. The carbohydrate might be conjugated with partialy denatured protein, therefore, it was difficult to eliminate them during extraction. Amino acid composition of okara protein was studied. The result showed that the essential amino acid compositions of okara protein and SPI were not different. The cysteine and threonine contents of okara protein were higher than those of SPI. The essential amino acid compositions and contents of okara protein were comparable to the FAO/WHO standard, with the exception of the cysteine, methionine and tyrosine which usually were lower in the soy proteins.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
เอกสารแนบ: http://www.lib.kmitl.ac.th
เผยแพร่โดย: สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหารลาดกระบัง
คำสำคัญ: วิทยานิพนธ์
เจ้าของลิขสิทธิ์: สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหารลาดกระบัง
รายละเอียด: Study on the interaction between moisture content of okara (4%, 8% and fresh >80%) and extraction time (1 and 2 hours at 38ฐC) on extracted protein yield was done. The result showed that extracted protein yield of okara which was dried at 60ฐC untill their moisture reached 4% and 8% were not different from that of fresh okara. Extracted protein yield were increased if extraction time increased from 1 hr to 2 hr The functional properties of protein would be effected by heating process (extraction and drying). Surface hydrophobicity (So) of okara protein extracted at various conditions was elucidated. So of okara protein was increased when increasing in extraction time and drying time. Protein would be partialy denatured and their structure would be unfolded during heating. The unfolding protein would expose buried hydrophobic groups to the surface of molecule. Okara protein extracted from fresh okara and used extraction time of 1 hr had So and Soulubility higher than other samples. The result found that there were significant interaction between moisture content of okara and extraction time on the emulsion activity and water holding capacity (WHC) of okara protein. WHC of okara protein extracted from dried okara was lower than those of wet okara. If the extraction time was increased from 1 to 2 hr, WHC of okara protein would be decreased. However, fat binding capacity (FBC) of okara protein would be increased if extraction time was increased from 1 to 2 hr. Moreover, foaming stability was effected by drying process. Foaming stability of okara protein that extracted from dried okara was better than that of fresh okara. Functional properties of okara protein and conventional soy protein isolate (SPI) were compared. The result showed that there was no different in funtional properties of okara protein and SPI. However, Foaming activity and FBC of okara protein were better than those of SPI. Subunit of okara protein was determined by using SDS-PAGE in the presence of 2-Mercaptoethanol. The result showed that subunit of okara protein was looked like subunits of SPI. Moreover, subunits of okara protein extracted at various experimental conditions were not different. The chemical composition of okara protein was also elucidated. The approximate analysis showed that okara protein contained 56-60% protein, 38-40% carbohydrate and 3-4% ash. The carbohydrate might be conjugated with partialy denatured protein, therefore, it was difficult to eliminate them during extraction. Amino acid composition of okara protein was studied. The result showed that the essential amino acid compositions of okara protein and SPI were not different. The cysteine and threonine contents of okara protein were higher than those of SPI. The essential amino acid compositions and contents of okara protein were comparable to the FAO/WHO standard, with the exception of the cysteine, methionine and tyrosine which usually were lower in the soy proteins.
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง


TARR Wordcloud:
การสกัดและการศึกษาคุณลักษณะของโปรตีนจากโอคารา.
สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหารลาดกระบัง
2545
การทำให้บริสุทธิ์ของโปรตีนจาก Bacillus subtilis N3 ที่มีฤทธิ์ต้านราก่อโรคพืช Bipolaris sp. ผลของอาหารที่มีโปรตีนต่ำต่อผลผลิตไก่พื้นเมือง การพัฒนาผลิตภัณฑ์น้ำพริกเผาเสริมโปรตีนและวิตามินบี 12 จากถั่วเหลืองหมัก. การผลิตและการศึกษาคุณลักษณะของโปรตีน พี53 ลูกผสมที่ การแยกและการศึกษาคุณสมบัติของเอ็นไซม์จากสัปะรดที่สามารถย่อยโปรตีนได้ การผลิตโปรตีนจากยีนส่วนนิวคลีโอโปรตีนของเชื้อไข้หวัดใหญ่ชนิดเอ ระดับโปรตีนและพลังงานที่เหมาะสมในสูตรอาหารสำหรับไก่ฟ้าคอแหวนที่ผลิตในเชิงการค้า การจำแนกโปรตีนเครื่องหมายเพื่อบ่งชี้ระยะการสุกแก่ของผลพลัมและแนคทารีนโดย HPLC การศึกษาการถ่ายยีนและการแสดงออกของยีนโปรตีนนมผึ้งในข้าวญี่ปุ่นพันธุ์ Kitaake และข้าวเหนียวพันธุ์ กข 6 สมบัติต้านเชื้อราของสารสกัดจากพืชบางชนิดต่อโรคที่เกิดจากเชื้อราในกุหลาบและเบญจมาศ
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก