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Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis
Jayashree R. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Jayashree R.
บทคัดย่อ (EN): Hevea brasillensis is a perennial tree and hence genetic improvement through conventional breeding is elaborate and time-consuming. Genetic transformation offers a viable approach to breeders supplementing the breeding programmes by adding valuable genes for specific characters in a relatively short period. The major objective of rubber breeding is to develop superior clones with improved dry rubber vield and wood quality. The HMGR (3-ydroxy 3-methylglutaryl Coenzyme A reductase) is considered as a key enzyme in the rubber biosynthetic pathway to transform the rubber plants for increased rubber yield. Agrobacterium tumefaciens mediated genctic transformation system is the most widely used method for Hevea genetic transformation because of its efficiency and convenience. The transfer of T-DNA and its integration into the plant genome is influenced by several factors. These include plant genotype, explant, plasmid vectors, bacterial strain, “vir” gene inducing phenolic compounds in the culture medium, culture media composition, suppression and elimination of Agrobacterium tumefaciens after co-cultivation etc. The selection of an appropriate bacterial strain is one of the key parameter required for an efficient genetic transformation system. The molecular and genetic basis for the host range of a bacterial strain is controlled by multiple factors within the bacterium and the host plant. Host range may further result from an interaction of particular Ti plasmids with certain bacterial chromosomal backgrounds. To identify a suitable bacterial strain giving efficient transformation with the hmgr1 gene in Hevea, three different Agrobacterium strains harbouring the transgene were experimented. Three Agrobacterium strains, EHA 105, LBA 4404 and pGV 1301 were evaluated for the efficiency of transformation in Hevea brasiliensis. The strain LBA 4404, a vir-helper, is an octopine type of bacterial strain, harboring disarmed Ti plasmid p AL 4404. The other two Agrobacterium strains EHA 105, a derivative of EHA 101 (Hood et al., 1993) and pGV 1301 which has a cured Ti plasmid, belong to the nopaline type. The binary vector contained hygromycin phosphotransferase gene (hpt) as the plant selectable marker) Gritz and Davies 1983). Three different target tissues, fresh callus, embryogenic callus and embryogenic suspension cultures were used for genetic transformation. The infected callus was incubated at different temperature regimes, 4°C, 20° and 28°C in the co-culture medium. Effective selection of the transformants was carried out in the screening medium supplemented with hygromycin (30mg lˉ¹). The transformants were selected and proliferated. The presence of the transgene was confirmed in the cell lines by PCR using gene specific primers. A clear distinction in the efficiencies between the Agrobacterium strains was observed when the culture was performed at 20°C for 3 days. Highest transformation efficiency was observed with the strain EHA 105 (28%), irrespective of the target tissues tried for transformation. The PCR confirmed cell lines were further cultured for embryo induction and subsequent plant regeneration.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): transformation
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis
Jayashree R.
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis CLONAL VARIABILITY IN THE INCLINATION OF LATICIFERS AND PHLOIC RAYS IN HEVEA BRASILIENSIS IMPROVEMENT AND APPLICATION OF THE TECHNIQUE OF MINI-SEEDLING BUDDING OF HEVEA BRASILIENSIS THE YIELD AND QUALITY OF RUBBER (HEVEA BRASILIENSIS) AS AFFECTED BY IRRIGATION AND FERTILIZER APPLICATIONS Investigation of Transcription Factor bHLH in Hevea DEVELOPMENT OF DWARF CLONES OF HEVEA BRASILIENSIS Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) A Mini Juvenile Bud Stick and Mini Seedling Budding of Hevea brasiliensis Seasonal Variation and Evolution of Some Latex Physiological Parameters of Hevea brasiliensis over Consecutive Tapping Years
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