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An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon
Kanjanasopa D. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon
ผู้แต่ง / หัวหน้าโครงการ (EN): Kanjanasopa D.
บทคัดย่อ (EN): Monodon baculovirus (MBV) causes slow growth of the shrimp being due to its infection in the hepatopancreas, the organ producing digestive enzymes and for nutrient storage. It has long been documented that the virus is transmitted from broodstock to offspring via contamination in rearing water by feces of the infected broodstock that contained sloughing-off damaged hepatopancreatic cells containing MBV. As a management practice, the washing of eggs and nauplii with disinfectant has been recommended to eliminate MBV that are attached to their surface and thus prevent the infection at the later development stage of the shrimp. However, we detected the MBV infection in postlarvae and juveniles of the black tiger shrimp Penaeus monodon, even after our repeated attempts to eliminate the virus by washing the eggs and nauplii with povidone iodine as disinfectant. Therefore we used MBV-infected broodstock to identify the cause of this problem by tracing the presence of MBV in hepatopancreas and ovary of the broodstock, eggs, nauplii and postlarvae, using histology with hematoxylin-eosin staining, polymerase chain reaction (PCR), in situ hybridization (ISH) and immunohistochemistry (IHC) specific for polyhedrin, a protein produced by MBV. We found all the ovaries collected from the broodstock, which were detected MBV-positive in the hepatopancreas by histology, PCR and ISH, were also PCR-positive. By ISH, positive signals were detected in the cell membrane, cytoplasm and nuclear membrane of the oocytes. The eggs and nauplii from the MBV-positive broodstock were also positive by PCR, in both "wash" and "no-wash" specimens. By ISH, positive signals were detected in ooplasm and subcuticular region of nauplii, as well as inside its body. Using IHC, positive signals were detected inside the body and appendage of the nauplius. Taken all these together, it is most likely that MBV could be vertically transmitted through trans-ovarian route. Hence, simply washing the eggs and nauplii with disinfectant may not be an adequate procedure to eliminate the MBV infection in offspring from MBV-infected broodstock. © 2015 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925015122&doi=10.1016%2fj.aquaculture.2015.02.041&partnerID=40&md5=f81b586c4b5d499735abeaa8b70e2fcc
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Penaeus monodon
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Kanjanasopa D.. "An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kanjanasopa D.. "An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon
Kanjanasopa D.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Evidence that the whiteleg shrimp Penaeus (Litopenaeus) vannamei is refractory to infection by Penaeus monodon nudivirus (PmNV), also known as MBV Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon Correlation between gonad-inhibiting hormone and vitellogenin during ovarian maturation in the domesticated Penaeus monodon Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain Suppression of PmRab11 inhibits YHV infection in Penaeus monodon Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon)
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