คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Wongwajana

เผยแพร่โดย

1 Department of Microbiology, and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. 2 International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh. 3 Research Group for Preventive Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand. 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand. 5 Department of Microbiology, and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: chariya@kku.ac.th.

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand Mangrove forests in Thailand Characterisation of Classical Enterotoxins, Virulence Activity, and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Thai Fermented Pork Sausages, Clinical Samples, and Healthy Carrier Characterization and genetic variation of vibrio cholerae isolated from clinical and environmental sources in Thailand Progress on Rubber Breeding of Thailand การเฝ้าระวัง Vibrio parahaemolyticus, Staphylococcus aureus และ Salmonella spp. ในเนื้อปูต้มแกะ / Surveillance of Vibrio parahaemolyticus, Staphylococcus aureus and Salmonella spp. in Boiled Crab Meat

คัดลอก URL

ชื่อเรื่อง (EN):	Serogroup, virulence, and molecular traits of Vibrio parahaemolyticus isolated from clinical and cockle sources in northeastern Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Wongwajana
บทคัดย่อ (EN):	Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n=74) and cockles (Anadara granosa) (n=74) in Thailand were analyzed by serotyping, determination of virulence and related marker genes present, response to antimicrobial agents, and genetic relatedness. Serological analysis revealed 31 different serotypes, 10 of which occurred among both clinical and cockle samples. The clinical isolates commonly included the pandemic serogroup O3:K6, while a few of the cockle isolates exhibited likely pandemic serovariants such as O3:KUT and O4:KUT, but not O3:K6. The pandemic (orf8 gene-positive) strains were more frequently found among clinical isolates (78.4%) than cockle isolates (28.4%) (p<0.001). Likewise, the virulence and related marker genes were more commonly detected among clinical than cockle isolates; i.e., tdh gene (93.2% versus 29.7%), vcrD2 (97.3% versus 23.0%), vopB2 (89.2% versus 13.5%), vopT (98.6% versus 36.5%) (all p<0.001) and trh (10.8% versus 1.4%) (p<0.05). Pulsed-field gel electrophoresis of NotI-digested genomic DNA of 41 randomly selected V. parahaemolyticus isolates representing different serotypes produced 33 pulsotypes that formed 5 different clusters (clonal complexes) (A-E) in a dendrogram. Vibrio parahaemolyticus O3:K6 and likely related pandemic serotypes were especially common among the numerous clinical isolates in cluster C, suggesting a close clonal link among many of these isolates. Most clinical and cockle isolates were resistant to ampicillin. This study indicates that O3:K6 and its likely serovariants based on the PFGE clusters, are causative agents. Seafoods such as cockles potentially serve as a source of virulent V. parahaemolyticus, but further work is required to identify possible additional sources.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=496
เผยแพร่โดย (EN):	1 Department of Microbiology, and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. 2 International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh. 3 Research Group for Preventive Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand. 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand. 5 Department of Microbiology, and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: chariya@kku.ac.th.
คำสำคัญ (EN):	Vibrio parahaemolyticus Serogroup Clinical sourc eCockle source Virulence gene Pulsed-field gel electrophoresis
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science