คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Uranan Tumkosit

เผยแพร่โดย

1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 3 Institute of Molecular Function, Misato, Saitama, Japan 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand

เผยแพร่เมื่อ ไม่ระบุวันที่เผยแพร่

หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Characterization of Chikungunya Virus-Like Particles Development and evaluation of multiplex real-time RT-PCR for Dengue, Chikungunya and Zika virus detection in Thailand Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development Characterization of chikungunya virus-like particles Generation and characterization of cross neutralizing human monoclonal antibody against 4 serotypes of dengue virus without enhancing activity Dengue, Japanese encephalitis and Chikungunya virus antibody prevalence among captive monkey (Macaca nemestrina) colonies of Northern Thailand Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method Viral infections in tilapines: More than just tilapia lake virus Parasite and viral species richness of Southeast Asian bats: Fragmentation of area distribution matters Monitoring arbovirus in Thailand: Surveillance of dengue, chikungunya and zika virus, with a focus on coinfections

คัดลอก URL

ชื่อเรื่อง (EN):	Anti-Chikungunya Virus Monoclonal Antibody That Inhibits Viral Fusion and Release
ผู้แต่ง / หัวหน้าโครงการ (EN):	Uranan Tumkosit
บทคัดย่อ (EN):	Chikungunya fever, a mosquito-borne disease manifested by fever, rash,myalgia, and arthralgia, is caused by chikungunya virus (CHIKV), which belongs to the genus Alphavirus of the family Togaviridae. Anti-CHIKV IgG from convalescent patients is known to directly neutralize CHIKV, and the state of immunity lasts throughout life. Here, we examined the epitope of a neutralizing mouse monoclonal antibody against CHIKV, CHE19, which inhibits viral fusion and release. In silico docking analysis showed that the epitope of CHE19 was localized in the viral E2 envelope and consisted of two separate segments, an N-linker and a _-ribbon connector, and that its bound Fab fragment on E2 overlapped the position that the E3 glycoprotein originally occupied. We showed that CHIKV-E2 is lost during the viral internalization and that CHE19 inhibits the elimination of CHIKV-E2. These findings suggested that CHE19 stabilizes the E2-E1 heterodimer instead of E3 and inhibits the protrusion of the E1 fusion loop and subsequent membrane fusion. In addition, the antigen-bound Fab fragment configuration showed that CHE19 connects to the CHIKV spikes existing on the two individual virions, leading us to conclude that the CHE19-CHIKV complex was responsible for the large virus aggregations. In our subsequent filtration experiments, large viral aggregations by CHE19 were trapped by a 0.45 µm filter. This virion-connecting characteristic of CHE19 could explain the inhibition of viral release from infected cells by the tethering effect of the virion itself. These findings provide clues toward the development of effective prophylactic and therapeutic monoclonal antibodies against the Alphavirus infection.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=760
เผยแพร่โดย (EN):	1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 3 Institute of Molecular Function, Misato, Saitama, Japan 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand
คำสำคัญ (EN):	Viral membrane fusion
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science