คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Warawan Wongboot

เผยแพร่โดย

1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases, Osaka University, Osaka, Japan 3 National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand

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งานวิจัยที่แนะนำ Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Emergence and Characterization of Unusual DS-1-Like G1P[8] Rotavirus Strains in Children with Diarrhea in Thailand Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand Sufficient Weapons for control of Mosquitoes and Other Vectors Dengue Encephalitis Filariasis Fevers Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand Molecular detection and characterization of noroviruses in river water in Thailand Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010

คัดลอก URL

ชื่อเรื่อง (EN):	Detection, identification and characterization of enteric bacterial pathogens in a case-control study of acute diarrhea in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Warawan Wongboot
บทคัดย่อ (EN):	Diarrheal disease is a crucial public health issue in Thailand and globally. We conducted two-year pathogen surveillance based on a case-control study to determine etiology of acute diarrhea in Thailand. Here, we present the results of detection and identification of bacterial pathogens in diarrheal and non-diarrheal stools. Stool samples were collected from 370 hospitalized patients with acute diarrhea and 370 non-diarrheal controls and those samples were tested for the presence of enteropathogens by a multiplex quantitative PCR (qPCR) panel assay and bacterial culture methods. Some isolates of enteropathogens were further confirmed or characterized by PCR and serotyping. Bacterial enteropathogen(s) including Aeromonas, Campylobacter, diarrheagenic E. coli, Plesiomonas, Yersinia, Salmonella, Shigella and Vibrio were detected in 314 cases (84.9%) and 310 controls (83.8%) using qPCR assays, whereas by culture methods 234 and 230 bacterial enteropathogens were isolated from 170 cases (45.9%) and 169 controls (45.7%), respectively. Two or more possible bacterial pathogens were isolated in 48 cases (13.0%) and 47 controls (12.7%). The most common bacteria were E. coli (31.4% vs 37.0%), Salmonella (8.9% vs 8.9%), and Aeromonas (5.9% vs 4.3%) in both cases and controls. The isolation rates of Shigella and Campylobacter species were statistically associated with diarrhea, and S. sonnei and C. jejuni were the dominant species. Further, 306 isolates of E. coli were classified into 42 genotypes by multiplex-PCR assays and ≥ 8 different O-serotypes were identified among 66 Salmonella isolates. Most bacterial pathogens were similarly distributed in both patients and control subjects. Isolation rates of enteropathogens in cases and controls were no significantly different except for Campylobacter and Shigella. The issue of high rates of asymptomatic carriage and multiple infections in patients highlighted the complexity of determining the causative agent of acute diarrhea by culture methods in this study site.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=696
เผยแพร่โดย (EN):	1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases, Osaka University, Osaka, Japan 3 National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand
คำสำคัญ (EN):	Thailand
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science