สืบค้นงานวิจัย
Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source
Chang Y.C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source
ผู้แต่ง / หัวหน้าโครงการ (EN): Chang Y.C.
บทคัดย่อ (EN): The ability to grow on media containing certain D-amino acids as a sole nitrogen source is widely utilized to differentiate Cryptococcus gattii from C. neoformans. We used the C. neoformans H99 and C. gattii R265 strains to dissect the mechanisms of D-amino acids utilization. We identified three putative D-amino acid oxidase (DAO) genes in both strains and showed that each DAO gene plays different roles in D-amino acid utilization in each strain. Deletion of DAO2 retarded growth of R265 on eleven D-amino acids suggesting its prominent role on D-amino acid assimilation in R265. All three R265 DAO genes contributed to growth on D-Asn and D-Asp. DAO3 was required for growth and detoxification of D-Glu by both R265 and H99. Although growth of H99 on most D-amino acids was poor, deletion of DAO1 or DAO3 further exacerbated it on four D-amino acids. Overexpression of DAO2 or DAO3 enabled H99 to grow robustly on several D-amino acids suggesting that expression levels of the native DAO genes in H99 were insufficient for growth on D-amino acids. Replacing the H99 DAO2 gene with a single copy of the R265 DAO2 gene also enabled its utilization of several D-amino acids. Results of gene and promoter swaps of the DAO2 genes suggested that enzymatic activity of Dao2 in H99 might be lower compared to the R265 strain. A reduction in virulence was only observed when all DAO genes were deleted in R265 but not in H99 indicating a pathobiologically exclusive role of the DAO genes in R265. These results suggest that C. neoformans and C. gattii divergently evolved in D-amino acid utilization influenced by their major ecological niches. © 2015, Public Library of Science. All rights reserved. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938883780&doi=10.1371%2fjournal.pone.0131865&partnerID=40&md5=3ac0f3818dedb330d9834507b1362006
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Virulence
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Chang Y.C.. "Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chang Y.C.. "Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source
Chang Y.C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex Molecular genetics database of poisonous mushrooms and sustainable utilization Soil Nitrate as a Source of Nitrogen for Rainfed and Deep-Flooded Rice Utilization of Waste of Rubber Plantation and Ccrumb Rubber Factory as a Source of Bioenergy Energy and nitrogen utilization of Thai native cattle given low quality hay and sago palm pith with different levels of soybean meal Use of lianas by primates: more than a food source Recent Status of Rubberwood Utilization in Cambodia Molecular diagnosis of α-thalassemias by the colorimetric nanogold Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair