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Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp
Phetrungnapha A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp
ผู้แต่ง / หัวหน้าโครงการ (EN): Phetrungnapha A.
บทคัดย่อ (EN): Identification and characterization of the RNAi-related genes is the key to understanding RNAi mechanism in shrimp. In this study, we have identified and characterized a novel putative RNA helicase gene, Mj-mov-10 from the kuruma shrimp, Marsupenaeus japonicus and its implication in shrimp RNAi was demonstrated. The full-length Mj-mov-10 gene contained 3536. bp, including 239bp of 5'UTR, 2895bp of the open reading frame (ORF) and 402. bp of 3'UTR, respectively. An ORF of Mj-mov-10 could be translated to a 109-kDa protein which consists of a single helicase core domain containing seven signature motifs of the RNA helicase superfamily-1. Mj-MOV-10 protein shared 47% and 40% identity with mammalian MOV-10 and plant SDE3, respectively. Expression of Mj-mov-10 gene was significantly up-regulated upon dsRNA and white spot syndrome virus (WSSV) challenge. Invivo gene knockdown of Mj-mov-10 resulted in an increase of a susceptibility of shrimp to WSSV infection. Our results implied the functional significance of Mj-MOV-10 in dsRNA-mediated gene silencing and antiviral defense mechanism in shrimp. © 2015 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924275420&doi=10.1016%2fj.fsi.2015.02.028&partnerID=40&md5=6270cd6cea92e3cd56bb16343062d564
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): RNA Interference
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp
Phetrungnapha A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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