สืบค้นงานวิจัย
Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis
Zhiying Cai - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis
ผู้แต่ง / หัวหน้าโครงการ (EN): Zhiying Cai
บทคัดย่อ (EN): To gain more insight into the molecular mechanisms of Colletotrichum gloeosporioides pathogenesis, Agrobacterium tumefaciens-mediated transformation (ATMT) was used to identify mutants of C. gloeosporioides impaired in pathogenicity. An ATMT library of 4128 C. gloeosporioides transformants was generated. Transformants were screened for defects in pathogenicity with a detached copper brown leaf assay. 32 mutants showing reproducible pathogenicity defects were obtained. Southern blot analysis showed 60.4% of the transformants had single-site T-DNA integrations. 16 Genomic sequences flanking T-DNA were recovered from mutants by thermal asymmetric interlaced PCR, and were used to isolate the tagged genes from the genome sequence of wild-type C. gloeosporioides by Basic Local Alignment Search Tool searches against the local genome database of the wild-type C. gloeosporioides. One potential pathogenicity genes encoded calcium-translocating P-type ATPase. Six potential pathogenicity genes had no known homologs in filamentous fungi and were likely to be novel fungal virulence factors. Two putative genes encoded Glycosyltransferase family 28 domain-containing protein and Mov34/MPN/PAD-1 family protein, respectively. Five potential pathogenicity genes had putative function matched with putative protein of other Colletotrichum species. Two known C. gloeosporioides pathogenicity genes were also identified, the encoding Glomerella cingulata hard-surface induced protein and C. gloeosporioides regulatory subunit of protein kinase A gene involved in cAMP-dependent PKA signal transduction pathway.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.sciencedirect.com/science/article/pii/S0944501313000062/pdfft?md5=e776f265b0712cf81b1f9353c3a67ea9&pid=1-s2.0-S0944501313000062-main.pdf
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): molecular mechanisms
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Zhiying Cai. "Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Zhiying Cai. "Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis
Zhiying Cai
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
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