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Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava
Phookaew P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava
ผู้แต่ง / หัวหน้าโครงการ (EN): Phookaew P.
บทคัดย่อ (EN): Cassava (Manihot esculenta Crantz) is an important crop and it is significantly affected by water stress. The computational analysis of cis-regulatory elements in promoter regions of 21 drought-responsive miRNA gene families and 35 miRNA-target genes in cassava indicated some elements relevant to drought stress responses. To investigate the role of miRNAs and target genes in responses to a water deficit in cassava in more detail, in vitro plantlets were subjected to an imitated water deficit by 40 % polyethylene glycol. Using RT-qPCR, the differential expression of the cassava miR164/MesNAC and miR167/MesARF6/8 were observed to be associated with changes in the leaf shape, stomatal closure, and relative water content. The modified 5′-RNA ligase-mediated rapid amplification of cDNA-end (5'RLM-RACE) experiment confirmed MesNAC and MesARF8 as the in vivo-target genes of miR164 and miR167, respectively, in cassava leaf. The possible functions of miR164 and miR167-target genes in response to water deficit are discussed. © 2014 Springer Science+Business Media Dordrecht.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906078606&doi=10.1007%2fs10535-014-0410-0&partnerID=40&md5=dea6fd76d1f5461a39c59707c331b0ce
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Manihot esculenta
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava
Phookaew P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Genetic Linkage Map of Cassava (Manihot esculenta Crantz) Based on Rubber Tree and Cassava Simple Sequence Repeat Markers Isolation and Characterization of Microsatellite Loci and Genetic Diversity in Cassava (Manihot esculenta Crantz) Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray Quantitative trait loci underlying root yield and starch content in an F1 derived cassava population (Manihot esculenta Crantz) Involvement of miR160/miR393 and their targets in cassava responses to anthracnose disease Phenotypic variation of cassava root traits and their responses to drought Genome-wide analysis of aquaporin gene family and their responses to water-deficit stress conditions in cassava Physio-morphological responses of sweet potato [Ipomoea batatas (L.) Lam.] genotypes to water-deficit stress Dissection of gene loci underlying pasting temperature in cassava Gene co-expression analysis inferring the crosstalk of ethylene and gibberellin in modulating the transcriptional acclimation of cassava root growth in different seasons
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