สืบค้นงานวิจัย
Ethylene induces Lipoxygenase Genes (jasmonate pathway) early upregulation in the inner bark tissues of Rubber Tree
T. Rotchanapreeda - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Ethylene induces Lipoxygenase Genes (jasmonate pathway) early upregulation in the inner bark tissues of Rubber Tree
ผู้แต่ง / หัวหน้าโครงการ (EN): T. Rotchanapreeda
บทคัดย่อ (EN): Ethylene (Ethrel) is used in rubber plantations to increase rubber yield. Ethylene is a phytohormone involved in regulations of plant growth and development, including stress and defense responses. The effects of ethylene to increase the natural rubber yield have been extensively investigated for its physiological role in the laticifers. However, the molecular events of ethylene action in the inner bark tissues of mature rubber tree have not been extensively studied yet. In this study, we used the Suppression Subtractive Hybridization (SSH) technique to monitor the differentially expressed genes in the inner bark, between ethylene-treated and non-treated (control) rubber trees (2-years tapping). Among the sequenced 2,231-ESTs from these bark SSH libraries (ethylene vs control and control vs ethylene), 55 ESTs, grouped in 4 contigs, identified as at least two different isoforms of plant lipoxygenase, were present in the ethylene-treated specific bark SSH library only (no LOX EST was found in our latex SSH libraries), indicating probable up-regulation of these bark LOX genes in response to ethylene. Lipoxygenases are involved in the biosynthesis pathway of jasmonic acid (JA), a plant regulator involved in plant development and produced in systemic response to wounding and pathogens, thus playing an important role to mediate defense response genes. Results from semi-quantitative RTPCR confirmed that the expression of these lipoxygenase gene isoforms was very low or undetectable in the inner bark of the control trees, but were transiently markedly induced in Ethrel-treated rubber tree bark only. In the bark, LOX expression was maximum as soon as 4 h and until 8h after the treatment, then decreased progressively from 16 h and return almost to its basic level at 40 h. Using the same specific primers neither any basal expression nor ethylene induced overexpression of LOX could be detected in the latex, indicating that these LOX gene isoforms were tissue (bark) specific. These results confirm the well-known crosstalk between the ethylene and jasmonate pathways in plant.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): SSH library
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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T. Rotchanapreeda. "Ethylene induces Lipoxygenase Genes (jasmonate pathway) early upregulation in the inner bark tissues of Rubber Tree". ไม่ระบุหน่วยงาน. ม.ป.ป.

T. Rotchanapreeda. "Ethylene induces Lipoxygenase Genes (jasmonate pathway) early upregulation in the inner bark tissues of Rubber Tree". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Ethylene induces Lipoxygenase Genes (jasmonate pathway) early upregulation in the inner bark tissues of Rubber Tree
T. Rotchanapreeda
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis Hydrogen Peroxide is Essential for Wound-Induced Secondary Laticifer Differentiation via Activating Jasmonate Biosynthesis in Rubber Tree (Hevea brasiliensis Müll. Arg.) Chloroplast DNA Microsatellite Differentiation in Cultivated and Wild Rubber Tree (Hevea brasiliensis) Transcriptome Analyses Reveal Molecular Mechanism Underlying Tapping Panel Dryness of Rubber Tree (Hevea brasiliensis) The Role of Myanmar Rubber Planters and Producers Association (MRPPA) in Natural Rubber Development and its Recent Activities Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis) Tillage, Super Absorbent Polymer and Direct Seeding in Polybags can Mitigate Drought Effects During Early Stage of Rubber (Hevea brasiliensis) Plantation FEASIBILITY OF RUBBER (Hevea brasiliensis Mull. Arg.) CULTIVATION IN THE EASTERN PROVINCE OF SRI LANKA; A NON TRADITIONAL AREA FOR RUBBER Localization of Peroxidase Enzyme in the Bark of Hevea brasiliensis
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