สืบค้นงานวิจัย
Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp
Ananphongmanee V. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp
ผู้แต่ง / หัวหน้าโครงการ (EN): Ananphongmanee V.
บทคัดย่อ (EN): Cell surface display using the yeasts Saccharomyces cerevisiae and Pichia pastoris has been extensively developed for application in bioindustrial processes. Due to the rigid structure of their cell walls, a number of proteins have been successfully displayed on their cell surfaces. It was previously reported that the viral binding protein Rab7 from the giant tiger shrimp Penaeus monodon (PmRab7) and its binding partner envelope protein VP28 of white spot syndrome virus (WSSV) could independently protect shrimp against WSSV infection. Thus, we aimed to display these two proteins independently on the cell surfaces of 2 yeast clones with the ultimate goal of using a mixture of the two clones as an orally deliverable, antiviral agent to protect shrimp against WSSV infection. PmRab7 and VP28 were modified by N-terminal tagging to the C-terminal half of S. cerevisiae α-agglutinin. DNA fragments, harboring fused-gene expression cassettes under control of an alcohol oxidase I (AOX1) promoter were constructed and used to transform the yeast cells. Immunofluorescence microscopy with antibodies specific to both proteins demonstrated that mutated PmRab7 (mPmRab7) and partial VP28 (pVP28) were localized on the cell surfaces of the respective clones, and fluorescence intensity for each was significantly higher than that of control cells by flow cytometry. Enzyme-linked immunosorbant assay (ELISA) using cells displaying mPmRab7 or pVP28 revealed that the binding of specific antibodies for each was dose-dependent, and could be saturated. In addition, the binding of mPmRab7-expressing cells with free VP28, and vice versa was dose dependent. Binding between the two surface-expressed proteins was confirmed by an assay showing agglutination between cells expressing complementary mPmRab7 and pVP28. In summary, our genetically engineered P. pastoris can display biologically active mPmRab7 and pVP28 and is now ready for evaluation of efficacy in protecting shrimp against WSSV by oral administration. © 2015 Ananphongmanee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939159890&doi=10.1371%2fjournal.pone.0128764&partnerID=40&md5=e4560dfa63a3b619f59e6bb35daccca4
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): White spot syndrome virus 1
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Ananphongmanee V.. "Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp
Ananphongmanee V.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture Shrimp hemocyte homeostasis-associated protein (PmHHAP) interacts with WSSV134 to control apoptosis in white spot syndrome virus infection Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon ความสัมพันธ์ระหว่างสิ่งแวดล้อมและการเกิดโรคไวรัสตัวแดงดวงขาว (White spot syndrome Virus, WSSV) ในกุ้งกุลาดำ Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp ความสัมพันธ์ระหว่างปริมาณเชื้อไวรัสตัวแดงดวงขาว (White spot syndrome virus, WSSV) กับการถ่ายทอดโรคในกุ้งกุลาดำโดยใช้เทคนิค real time PCR Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon ICP35 is a TREX-like protein identified in white spot syndrome virus
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