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Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2))
Bharat Bhushan - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Bharat Bhushan
หน่วยงานสังกัดผู้แต่ง (EN): Uttar Pradesh (India). Indian Council of Agricultural Research. Division of Animal Genetics
บทคัดย่อ (EN): Characterization of major histocompatibility complex (MHC) Class I Exon 4-5 was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), single-strand confirmation polymorphism (SSCP) and sequencing techniques in crossbred cattle and buffaloes. Digestion of 559 bp fragment with HinfI and HaeIII restriction enzymes produced five and three distinct PCR-RFLP patterns in cattle and buffaloes, respectively. The genotype frequencies of HinfI patterns ranged from 0.015 to 0.456 in crossbred cattle, 0.080 to 0.600 in Mehsana and 0.050 to 0.500 in Bhadawari breeds of buffaloes. Similarly, the genotype frequencies of HaeIII patterns ranged from 0.045 to 0.636. The SSCP analysis showed that the BoLA-A and BuLA-A were highly polymorphic in cattle and buffaloes. A total of 10 alleles (AA, AB, BB, AC, AD, AE, AF, BC, DD and DB) were identified by PCR-SSCP. Four partial sequences, two each for crossbred cattle (AY790633 and AY790634) and buffaloes (AY785759 and AY785760), were submitted to the GenBank, NCBI. The sequencing results showed a number of amino acid changes in Exon 4-5 region. In conclusion, MHC Class I Exon 4-5 was found to be highly polymorphic in both cattle and buffaloes which may be exploited for association with traits of economic interest.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/410308
คำสำคัญ (EN): MHC
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Bharat Bhushan. "Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Bharat Bhushan. "Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2))
Bharat Bhushan
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3)) Surgical reconstruction of teat fistula in a buffalo (Buffalo Bulletin,40(1)) Pathological study of acute fasciolosis in a buffalo (Buffalo Bulletin,40(1)) Analysis of economic structure in water buffalo breeding by geographical regions in Turkey (Buffalo Bulletin,40(1)) Micrometrical observations of liver in buffalo (Bubalus bubalis) (Buffalo Bulletin,40(2)) Test-day genetic analysis of Murrah buffalo sires for milk production (Buffalo Bulletin (Thailand),35(1)) Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo (Buffalo Bulletin (Thailand),37(1)) An overview of traumatic reticulopericarditis in cattle and buffaloes (Buffalo Bulletin,40(2)) Cryptosporidiosis in buffalo calves (Buffalo Bulletin,39(2)) Promoter sequence analysis of ATP citrate lyase gene in buffalo and Vechur cattle (Buffalo Bulletin (Thailand),35(2))
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