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Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine (Buffalo Bulletin,41(3))
Reda Ibrahim El-Sheshtawy - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine (Buffalo Bulletin,41(3))
ผู้แต่ง / หัวหน้าโครงการ (EN): Reda Ibrahim El-Sheshtawy
หน่วยงานสังกัดผู้แต่ง (EN): Giza (Egypt). Veterinary Research Institute. Department of Animal Reproduction and Artificial Insemination
บทคัดย่อ (EN): Cryopreservation is accompanied with much detrimental premature sperm capacitation, decreased livability and fertility consequences. Taurine is considered as an antioxidant ameliorating the semen value post freeze-thawing. The purpose of this investigation was to elucidate the impact of Tris-diluent enriched with a mixture of taurine and different concentrations of butylated hydroxytoluene on buffalo bull semen preservability. Semen samples were extended in a Tris extender. Variable concentrations of butylated hydroxytoluene (BHT) (0.5, 1.0 and 2.0 mM) were set in ethyl alcohol in prewarmed (37 deg C) test tubes. Then taurine (60 mM) was added into each tube. Extended semen samples were added into the test tubes and put at 37 deg C for 5 minutes to permit the permeation of BHT into the spermatozoa. The control test tubes were Tris extender with (zero BHT and zero taurine). The test tubes were exposed to the freezing protocol. Semen was assessed and conception rate was carried out. The sperm membrane integrity (HOST) was eminently higher in TTB1 and TTB2 if compared to the control post cooling (Table 1). The post thawing results (Table 2) revealed significant improvement in sperm motility in TTB2 if compared to the control and other concentrations. Alive sperm percent was kept in all concentrations as the control. Sperm abnormalities were kept in TTB2 and TTB3 as the control. Sperm membrane integrity (HOST) was significantly improved in all significantly as compared to the control. The acrosomal integrity was kept in TTB1 and TTB2 concentrations the same as the control The conception percent (Table 3) was higher in TTB2. It could be concluded that, the post cooling results exhibited the best semen quality in TTB1and TTB2. The post -thawing results revealed that, most improved sperm parameters and conception rate were in TTB2.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/419611
คำสำคัญ (EN): Butylated hydroxytoluene
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Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine (Buffalo Bulletin,41(3))
Reda Ibrahim El-Sheshtawy
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Comparative efficacy of coconut water diluent with different semen extenders for cryopreservation of Nili Ravi buffalo bull semen (Buffalo Bulletin,41(3)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Effect of different rearing systems on behavioural responses to novel stimuli in Murrah buffalo calves (Buffalo Bulletin,41(4)) Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle (Buffalo Bulletin,41(2)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Morphometric study of slaughtered Murrah buffalo bull testis and its correlation with seminal attributes of epididymal semen (Buffalo Bulletin,41(4)) Seasonal variation in semen quality and conception rate of Jaffarabadi buffalo bulls (Bubalus bubalis) in India (Buffalo Bulletin,41(3)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1))
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