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Cholesterol supplementation in extender improves quality of frozen-thawed Nili-Ravi buffalo bull semen (Buffalo Bulletin (Thailand),35(2))
Akhter, S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Cholesterol supplementation in extender improves quality of frozen-thawed Nili-Ravi buffalo bull semen (Buffalo Bulletin (Thailand),35(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Akhter, S.
หน่วยงานสังกัดผู้แต่ง (EN): Pir Mehr Ali Shah Arid Agriculture University Rawalpindi (Pakistan). Department of Zoology:University of Gujrat (Pakistan). Hafiz Hayat Campus. Department of Zoology:Pir Mehr Ali Shah Arid Agriculture University Rawalpindi (Pakistan). Department of Wildlife Management:Pir Mehr Ali Shah Arid Agriculture University Rawalpindi (Pakistan). Department of Zoology:University of Gujrat (Pakistan). Hafiz Hayat Campus. Department of Zoology:Pir Mehr Ali Shah Arid Agriculture University Rawalpindi (Pakistan). Department of Zoology:Pir Mehr Ali Shah Arid Agriculture University Rawalpindi (Pakistan). Department of Zoology
บทคัดย่อ (EN): The study was designed to investigate the effect of cholesterol supplementation in extender on post-thaw quality of cryopreserved Nili-Ravi buffalo bull spermatozoa. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina (42°C) for five weeks ie., two ejaculates/ week and bull (replicate; n=30). Two consecutive ejaculates from each bull were mixed and processed for initial evaluation, semen from each bull was split into four aliquots and diluted (37°C) in tris-citric acid extender having cholesterol 0.0 (control), 5.0, 10.0 and 20.0 ng/mL Diluted semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. Cooled semen was filled in 0.5 ml French straws at 4°C, kept on liquid nitrogen vapours for 10 minutes. and plunged in liquid nitrogen for storage. Frozen semen was thawed after 24 h at 37°C for 30 seconds. Sperm progressive motility, plasma membrane integrity and viability were higher (P0.05) in extender containing 5.0 ng/mL of cholesterol. However, cholesterol did not provide any significant benefit for chromatin integrity of buffalo spermatozoa. In conclusion, cholesterol supplementation in extender at a concentration of 5.0 ng/mL improved the post-thaw quality of cryopreserved Nili-Ravi buffalo bull spermatozoa.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/344234
คำสำคัญ (EN): Cholesterol
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Cholesterol supplementation in extender improves quality of frozen-thawed Nili-Ravi buffalo bull semen (Buffalo Bulletin (Thailand),35(2))
Akhter, S.
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Effect of thioglycol on fertility of frozen Nili-Ravi buffalo semen (Buffalo Bulletin (Thailand),35(2)) Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull (Buffalo Bulletin (Thailand),35(1)) β(1,4)-Galactosyltransferase-I gene polymorphisms in Pakistani Nili Ravi buffalo (Buffalo Bulletin (Thailand),35(3)) Genomic selection of Nili-Ravi buffalo: A choice for buffalo breeders (Buffalo Bulletin (Thailand),35(4)) Quality characteristics of fermented buffalo beef sausage (Buffalo Bulletin (Thailand),35(4)) Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3)) Uterine didelphys in Nili Ravi buffalo-A case report (Buffalo Bulletin (Thailand),36(4)) Endoscopic diagnosis of megaesophagus in a buffalo (Buffalo Bulletin (Thailand),35(2)) Hydroallantois in a buffalo with fetal anasarca (Buffalo Bulletin (Thailand),35(4)) An unusual case of ideopathic fibrinous pericarditis in Nili-Ravi buffalo-A case report (Buffalo Bulletin (Thailand),35(3))
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