สืบค้นงานวิจัย
Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia
Razak M.R.M.A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia
ผู้แต่ง / หัวหน้าโครงการ (EN): Razak M.R.M.A.
บทคัดย่อ (EN): Malaysia has a national goal to eliminate malaria by 2020. Understanding the genetic diversity of malaria parasites in residual transmission foci can provide invaluable information which may inform the intervention strategies used to reach elimination targets. This study was conducted to determine the genetic diversity level of P. falciparum isolates in malaria residual foci areas of Sabah. Malaria active case detection was conducted in Kalabakan and Kota Marudu. All individuals in the study sites were screened for malaria infection by rapid diagnostic test. Blood from P. falciparum-infected individuals were collected on filter paper prior to DNA extraction. Genotyping was performed using merozoite surface protein-1 (MSP-1), merozoite surface protein-2 (MSP-2), glutamate rich protein (GLURP) and 10 neutral microsatellite loci markers. The size of alleles, multiplicity of infection (MOI), mean number of alleles (Na), expected heterozygosity (He), linkage disequilibrium (LD) and genetic differentiation (FST) were determined. In Kalabakan, the MSP-1 and MSP-2 alleles were predominantly K1 and FC27 family types, respectively. The GLURP genotype VI (751-800 bp) was predominant. The MOI for MSP-1 and MSP-2 were 1.65 and 1.20, respectively. The Na per microsatellite locus was 1.70. The He values for MSP-1, MSP-2, GLURP and neutral microsatellites were 0.17, 0.37, 0.70 and 0.33, respectively. In Kota Marudu, the MSP-1 and MSP-2 alleles were predominantly MAD20 and 3D7 family types, respectively. The GLURP genotype IV (651-700 bp) was predominant. The MOI for both MSP-1 and MSP-2 was 1.05. The Na per microsatellite locus was 3.60. The He values for MSP-1, MSP-2, GLURP and neutral microsatellites were 0.24, 0.25, 0.69 and 0.30, respectively. A significant LD was observed in Kalabakan (0.495, p<0.01) and Kota Marudu P. falciparum populations (0.601, p<0.01). High genetic differentiation between Kalabakan and Kota Marudu P. falciparum populations was observed (FST = 0.532). The genetic data from the present study highlighted the limited diversity and contrasting genetic pattern of P. falciparum populations in the malaria declining areas of Sabah. © Copyright: 2016 Mohd Abd Razak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962159027&doi=10.1371%2fjournal.pone.0152415&partnerID=40&md5=9e78305ea6bd2bd302c47198594dde6c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Protozoan Proteins
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Razak M.R.M.A.. "Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia". ไม่ระบุหน่วยงาน. ม.ป.ป.

Razak M.R.M.A.. "Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia
Razak M.R.M.A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Artemisinin-resistant Plasmodium falciparum malaria Prevalence of Plasmodium falciparum molecular markers of antimalarial drug resistance in a residual malaria focus area in Sabah, Malaysia Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the China-Myanmar border area Genetic diversity of the Plasmodium vivax multidrug resistance 1 gene in Thai parasite populations Quantifying connectivity between local Plasmodium falciparum malaria parasite populations using identity by descent Plasmodium falciparum field isolates from areas of repeated emergence of drug resistant malaria show no evidence of hypermutator phenotype Inhibition of merozoite invasion and transient de-sequestration by sevuparin in humans with Plasmodium falciparum malaria Hemoglobin e prevalence among ethnic groups residing in malaria-endemic areas of Northern Thailand and its lack of association with plasmodium falciparum invasion in vitro Combinatorial Genetic Modeling of pfcrt-Mediated Drug Resistance Evolution in Plasmodium falciparum IgE low affinity receptor (CD23) expression, Plasmodium falciparum specific IgE and tumor necrosis factor-alpha production in Thai uncomplicated and severe falciparum malaria patients
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