สืบค้นงานวิจัย
AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE
Kessarin Tungngoen - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE
ผู้แต่ง / หัวหน้าโครงการ (EN): Kessarin Tungngoen
บทคัดย่อ (EN): Latex flow, the first limiting factor of rubber yield, is linked to latex viscosity, which is inversely related to its water content. Further, yield stimulation with Ethrel has been shown to induce an early influx of water in the latex cells, leading to extension of the bark drainage and displacement areas. Furthermore, preliminary ecophysiological studies on rubber tree Trunk Phloem Necrosis (TPN) showed that the diseased trees exhibit higher water potential, indicating they are facing some water stress. All these data confirm the importance of water exchanges between the different organs and tissues of the rubber tree, especially between the laticifers and the phloemic tissues, they are embedded in. But water cannot freely cross the biological membranes. Aquaporins are channel proteins known, in all living species, to facilitate water fluxes across the cells membranes. Two major aquaporin families are known in plants: the plasma membrane aquaporins (referred as the Plasmalemma Intrinsic Proteins: PIPs) and the tonoplast aquaporins (Tonoplast Intrinsic Proteins: TIPs) To investigate the possible role of aquaporins in relation with rubber yield and bark physiological diseases, eight different full-length cDNAs encoding phloem and laticifer specific PIPs and TIPs were cloned and characterized in this study. The nucleotide sequences of all full-length cDNAs ranged from 1 to 1.4 kb and encoded different sizes of proteins varying from 25 to 31 kDa. The deduced amino acid sequences of proteins showed significant similarities to PIPs subfamily members from other plants. Northern blot analysis showed that the subclass PIP-1 and PIP-2 genes were overexpressed in the phloemic tissues of diseased trees. In contrast, the subclass TIP was significantly overexpressed in healthy trees. Quantitative real time PCR technique was used to elucidate the function of each individual aquaporin isoform. All of these isoforms showed up-regulation in the phloemic tissues of diseased and wounded rubber trees. Each isoform was differentially expressed in healthy trees depended on tested rubber tree clones. While HbB-TIP1 up-regulation in all clones, marked up-or down-regulation of HbB_TIP1 ; HbL_PIP1 ;3, HbB_TIP2;1 and HbL_PIP2;3 were observed. More details of aquaporin isoform and the effect of bark treatment with Ethrel to their expressions are under investigation to clarify their roles in the rubber trees.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Plasma Membrane Intrinsic Proteins (PIPs)
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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การอ้างอิง

Kessarin Tungngoen. "AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kessarin Tungngoen. "AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE
Kessarin Tungngoen
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Is impeded root growth related to the occurrence of rubber tree Trunk Phloem Necrosis (TPN)? Preliminary results from NE Thailand Recent advances on biotechnology in rubber tree Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation. Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis Differential Expression of Methyl Jasmonate-Responsive Genes Correlates with Laticifer Vessel Proliferation in Phloem Tissue of Rubber Tree (Hevea brasiliensis) RUBBER TREE TRUNK PHLOEM NECROSIS IN NORTHEAST THAILAND: 1-INVESTIGATIONS ON SOIL HETEROGENEITIES AND DISEASE DISTRIBUTION RUBBER TREE TRUNK PHLOEM NECROSIS (TPN) IN NORTHEAST THAILAND: 2, WITHIN-STAND CHARACTERISATION OF AFFECTED TREES Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. EVOLUTION OF SOIL FERTILITY AND ITS CORRELATION WITH RUBBER TREE LEAF NUTRITION IN RUBBER PLANTATION Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis
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