สืบค้นงานวิจัย
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions
Jaruthamsophon K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions
ผู้แต่ง / หัวหน้าโครงการ (EN): Jaruthamsophon K.
บทคัดย่อ (EN): Currently, there are three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions in Asian populations. To analyze available HLA-B∗15:02 screening methods, we compared four screening methods, including a multiplex PCR method, a nested PCR method, a LAMP method and our new in-house PCR-dot blot hybridization method. These methods were reviewed regarding their sensitivity, specificity, false positivity and technical considerations. Possible false positive (FP) alleles and genotypes were checked regarding the primers and probes designs, using the IMGT/HLA database. Expected FP rates in Asian populations were predicted using the Allele Frequencies Net Database. All methods had a sensitivity of more than 99.9%, although giving FP results to certain very rare alleles and genotypes. The multiplex PCR method was the only test that gave FP results to certain genotypes of HLA-B∗15:13, the allele which is prevalent in Southeast Asian populations. In conclusion, the nested PCR, LAMP and our in-house methods could be applied in various Asian populations, but the multiplex PCR, or any test with FP to HLA-B∗15:13, should be applied with caution in the Southeast Asian populations. © 2016 Jaruthamsophon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973632360&doi=10.1371%2fjournal.pone.0155907&partnerID=40&md5=d3568d2d196453523647d529c78074d0
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Polymerase Chain Reaction
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Jaruthamsophon K.. "Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions". ไม่ระบุหน่วยงาน. ม.ป.ป.

Jaruthamsophon K.. "Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions
Jaruthamsophon K.
มหาวิทยาลัยมหิดล
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