สืบค้นงานวิจัย
Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay
Chyerochana N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay
ผู้แต่ง / หัวหน้าโครงการ (EN): Chyerochana N.
บทคัดย่อ (EN): Bacteriophages of the Bacteroides fragilis strains HSP40 and RYC2056 are used as indicators of human-specific and general (non-host specific) fecal pollution in water bodies. However, conventional anaerobic cultivation methods require 1-2 days of incubation. To overcome this limitation, in this study, we developed a DNA-based method to simultaneously detect representative bacteriophages (B40-8 and B56-3) that infect B. fragilis strains HSP40 and RYC2056, respectively. Both phages yielded a 224-bp amplicon with the primer pair BT5414/BT5415, and an additional 152-bp PCR product was observed for B40-8 with the primer pair BT5579/BT5580. The detection limits for B40-8 and B56-3 were 10-5 and 10-4 ng of pure DNA, and 1 and 50 ng of DNA template when 5 and 5,000 PFU/mL were spiked into distilled water, respectively. The assay exhibited a higher sensitivity for sewage samples, with < 0.1 and 15 PFU/mL of phages infecting HSP40 and RYC2056, respectively. The assay did not produce false positive results for the Bacteroides phages PG76, HB13, and GA17 or for the enterococcal phages AIM06 and SR14. The assay also detected RYC2056 phages that were isolated from sewage samples and the phage B40-8 when it was spiked into raw sewage. Thus, the newly developed PCR assay demonstrated potential for the environmental monitoring of Bacteroides bacteriophages, decreasing the analysis time to a few hours. © 2018, Faculty of Environment and Resource Studies,Mahidol University. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042522230&partnerID=40&md5=5aef55487452af8f196c35d1a47fb531
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Bacteroides fragilis
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Chyerochana N.. "Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chyerochana N.. "Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay
Chyerochana N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Assessment of swine-specific bacteriophages of Bacteroides fragilis in swine farms with different antibiotic practices Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay Duplex PCR assay and in situ hybridization for detection of Francisella spp. and Francisella noatunensis subsp. orientalis in red tilapia Development of molecular approach based on PCR assay for detection of histamine producing bacteria Development of Zika virus detection method by real time RT-PCR for using in Thailand การตรวจหาเชื้อก่อโรคอุจจาระร่วงเฉียบพลัน 19 ชนิด ด้วยวิธี Quantitative real-time PCR Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand A nested PCR assay to avoid false positive detection of the microsporidian enterocytozoon hepatopenaei (EHP) in environmental samples in shrimp farms Development of botulinum toxin detection assay by antibody capture ELISA Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo (Buffalo Bulletin (Thailand),37(1))
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