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Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon
Posiri P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon
ผู้แต่ง / หัวหน้าโครงการ (EN): Posiri P.
บทคัดย่อ (EN): Yellow head virus (YHV) is a virulent pathogen in black tiger shrimp. It causes high mortality within a few days after infection. In this study, colocalization between YHV and P. monodon Rab5 (PmRab5) which is a small GTPase protein was visualized at 10 min to 3 h post-YHV challenge under a confocal microscope. The result indicated that PmRab5 plays a key role in the early stage of endocytosis, and is involved in YHV trafficking process. Molecular cloning showed that the open reading frame of PmRab5 is 633 bp encoding a putative protein of 210 amino acids with an estimated molecular weight of 23 kDa. PmRab5 contained all Rab-specific signature motifs: Rab family motif (RabF), Rab subfamily motif (RabSF), G-boxes and cysteine prenylation signal. Silencing of PmRab5 by specific dsRNA reduced YHV replication inside the cells. Furthermore, lack of PmRab5 expression exhibited a delay of shrimp mortality after YHV infection. The results demonstrated that PmRab5 is involved in YHV early endosomal trafficking, suggesting its role in the early step of infection. Statement of relevance: Silencing of PmRab5 may be applicable for inhibition of YHV. © 2016 Elsevier B.V..
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962550854&doi=10.1016%2fj.aquaculture.2016.03.026&partnerID=40&md5=7e09665221a79914925c1d8d6002449d
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Yellow head virus
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon
Posiri P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon) In vitro neutralization of yellow head virus infection in shrimp using recombinant PmYRP65 protein An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes The use of green fluorescent protein-tagged virus-like particles as a tracer in the early phase of chikungunya infection Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon Evidence that the whiteleg shrimp Penaeus (Litopenaeus) vannamei is refractory to infection by Penaeus monodon nudivirus (PmNV), also known as MBV
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