สืบค้นงานวิจัย
Bead array for Listeria monocytogenes detection using specific monoclonal antibodies
Karoonuthaisiri N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Bead array for Listeria monocytogenes detection using specific monoclonal antibodies
ผู้แต่ง / หัวหน้าโครงการ (EN): Karoonuthaisiri N.
บทคัดย่อ (EN): To develop a detection method for human pathogenic Listeria monocytogenes, novel specific antibodies were obtained from hybridoma libraries generated by using formalin-killed and heat-killed L.monocytogenes as immunogens. Several monoclonal antibodies found to be specific to Listeria spp. or L.monocytogenes were evaluated for their applicability as binders for bead array and sandwich ELISA for detection of L.monocytogenes in buffer and in 11 different food types. The bead array format consistently demonstrated lower detection limits and was less affected by interference from food matrices than the sandwich ELISA format. However, the obtained detection limits were not sufficient to satisfy the required standard for L.monocytogenes testing. Therefore, the international organization for standardization (ISO 11290-1:1996) methods for pre-enrichment and enrichment were employed to increase the bacteria numbers. When compared to the standard plating method, the bead array was able to detect the bacteria with the same accuracy even at the 1CFU level after only 24h of the enrichment period. In addition, Listeria-specific 3C3 and L.monocytogenes-specific 7G4 antibodies were successfully employed to construct a multiplex detection for Listeria, Salmonella and Campylobacter in a bead array format by combining with commercial Salmonella-specific and available Campylobacter-specific antibodies. © 2014 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907164516&doi=10.1016%2fj.foodcont.2014.07.049&partnerID=40&md5=a15aed6ac4acd8bdc64bf707f718c0ba
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Multiplex detection
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Karoonuthaisiri N.. "Bead array for Listeria monocytogenes detection using specific monoclonal antibodies". ไม่ระบุหน่วยงาน. ม.ป.ป.

Karoonuthaisiri N.. "Bead array for Listeria monocytogenes detection using specific monoclonal antibodies". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Bead array for Listeria monocytogenes detection using specific monoclonal antibodies
Karoonuthaisiri N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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