สืบค้นงานวิจัย
Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis)
Thakurdas Saha - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis)
ผู้แต่ง / หัวหน้าโครงการ (EN): Thakurdas Saha
บทคัดย่อ (EN): Numerous disease-resistance genes have been cloned and characterized in various plant species. Only a few of these reported genes are transcriptionally induced or have enhanced transcription upon pathogen infection. A set of transcriptionally induced sequences (RT-RGAs), analogous to plant resistance genes of NBS-LRR class was characterized from Hevea brasiliensis, a tree crop producing latex of commercial utility. PCR-based approach was adopted using degenerated primers based on the conserved motifs of NBS domains of known plant R-genes. Sequence characterization of RT-RGAs from Corynespora cassicola challenged rubber plants of clone RRll 105 was performed and consecutively 32 transcriptionally active diverged RT-RGAs were identified. A comparison of these RT-RGAs with the earlier isolated RGAs revealed that a large group of closely related genomic RGAs had no function against Corynespora leaf disease. They did not show perfect homology with any of the RT-RGAs on the basis of deduced amino acid sequences. Similarly, tow groups consisting of 7 and 15 RT-RGAs were found to be unique as they did not share any homology with the genomic RGAs but were functionally active against Corynespora infection. Nucleotide sequence information of RT-RGA 13, one of the functional resistance gene analogues identified in rubber, showing significant over-expression in Corynespora infected rubber clones was used to derive its full-length sequence. Subsequently, a full-length resistance ® gene from rubber )GT1) was cloned and characterized for the first time. Length of the R gene was 3284 bp and the coding sequences was 2547 bp. Conceptual translation of the coding sequences revealed a protein of 849 aminoacids, which had characteristic NB-ARC domain and leucine-rich repeat (LRR) motif present in most of the R gene from other species and maximum homology was found with nbs-lrr resistance protein of Populus tricocarpa (E-value 0.0). Protein structure prediction was performed with comceptual translation of the R gene through homology modeling. Domain analysis resulted in highest match with apoptotic protease activation factor. Besides above, characteristic domains viz. coiled coil, leucine-rich repeat. Toll-like receptor2 also identified in the deduced R protein. The involvement of R gene in disease tolerance was assessed through real-time quantification of gene expression after challenging tolerant (GT 1) and susceptible (RRll105) clones of rubber with Corynespora cassiicola. RNA was isolated from the leaves collected at different time intervals (0, 6, 12 and 24 hours) following inoculation and converted to cDNA for real time quantification of the expression using specific primer-pairs. Differential expression of the R gene was noticed between these tow clones even in unchallenged condition (control) and expression level was significantly higher in tolerant clone GT 1. However, at 6 hours following infection, the expression levels reduced considerably in both the clones. At 12 hours, expression level of R gene was found to reduce drastically (5 fold compared to the expression level at 0 hour) in case of RRll 105, whereas expression shot up in GT1 reaching initial level of expression at 0 hour. However, at 24 hours expression level increased in RRll 105 by 30% but decreased in GT 1 by 50% of the expression level noticed at 12 hours following infection in respective clones. From this observation it is evident that increased level of R gene expression in GT 1 (8 folds more than in RRll 105) at 12 hours when pathogen establishes infection on host. May be one of the reason for tolerance to Corynespora cassiicoila observed in GT1.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Corynespora Cassiicola
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Thakurdas Saha. "Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.

Thakurdas Saha. "Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis)
Thakurdas Saha
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Identification of Resistance of Hevea brasiliensis Clones to Corynespora Leaf Fall Disease Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis) Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis) CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN The Effect of Weeding and Fungicide Treatments on the Development of Corynespora Leaf Fall Disease in Rubber Environment and Farmer Friendly Biodegradable Rubber Spray Oil for Controlling Abnormal Leaf Fall Disease Frequency of Occurrence and the Role of Colletotrichum Species on the Colletotrichum Leaf Fall Disease of Hevea brasiliensis in the Traditional Rubber Growing Regions of Kerala THE YIELD AND QUALITY OF RUBBER (HEVEA BRASILIENSIS) AS AFFECTED BY IRRIGATION AND FERTILIZER APPLICATIONS
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