สืบค้นงานวิจัย
Effect of iron overload on furin expression in wild-type and β-thalassemic mice
Wichaiyo S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Effect of iron overload on furin expression in wild-type and β-thalassemic mice
ผู้แต่ง / หัวหน้าโครงการ (EN): Wichaiyo S.
บทคัดย่อ (EN): Furin is a proprotein convertase enzyme. In the liver, it cleaves prohepcidin to form active hepcidin-25, which regulates systemic iron homeostasis. Hepcidin deficiency is a component of several iron overload disorders, including β-thalassemia. Several studies have identified factors that repress hepcidin gene transcription in iron overload. However, the effect of iron overload on furin, a post-translational regulator of hepcidin, has never been evaluated. The present study aimed to investigate the changes in furin and related factors in parenteral iron-overloaded mice, including those with β-thalassemia. Wild-type (WT) and β-thalassemia intermedia (th3/+) C57BL/6 mice were intraperitoneally injected with 9 doses of iron dextran (1. g iron/kg body weight) over 2 weeks. In the iron overload condition, our data demonstrated a significant Furin mRNA reduction in WT and th3/+ mice. In addition, the liver furin protein level in iron-overloaded WT mice was significantly reduced by 70% compared to control WT mice. However, the liver furin protein in iron-overloaded th3/+ mice did not show a significant reduction compared to control th3/+ mice. The hepcidin gene (hepcidin antimicrobial peptide gene, Hamp1) expression was increased in iron-overloaded WT and th3/+ mice. Surprisingly, the liver hepcidin protein level and total serum hepcidin were not increased in both WT and th3/+ mice with iron overload, regardless of the increase in Hamp1 mRNA. In conclusion, we demonstrate furin downregulation in conjunction with Hamp1 mRNA-unrelated pattern of hepcidin protein expression in iron-overloaded mice, particularly the WT mice, suggesting that, not only the amount of hepcidin but also the furin-mediated physiological activity may be decreased in severe iron overload condition. © 2015 The Authors.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925507151&doi=10.1016%2fj.toxrep.2015.01.004&partnerID=40&md5=25a8b1587874373ebccc0002a820eefe
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Western blotting
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Wichaiyo S.. "Effect of iron overload on furin expression in wild-type and β-thalassemic mice". ไม่ระบุหน่วยงาน. ม.ป.ป.

Wichaiyo S.. "Effect of iron overload on furin expression in wild-type and β-thalassemic mice". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Effect of iron overload on furin expression in wild-type and β-thalassemic mice
Wichaiyo S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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