คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Akatsuki Saito

เผยแพร่โดย

1 Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. 2 Thailand-Japan Research Collaboration Center on Emerging and Re-Emerging Infections (RCC-ERI), Osaka University, Nonthaburi,Thailand. 3 National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi, Thailand.

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งานวิจัยที่แนะนำ Optimization of genotyping by phenotypic analysis of clinical HIV-1 subtype CRF01_AE isolates from South-East Asia Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand External Quality Assessment Scheme for HIV-1 Drug Resistance Genotyping in Thailand Hepatitis B and C co-infection in HIV patients from the TREAT Asia HIV observational database: Analysis of risk factors and survival Glycans flanking the hypervariable connecting peptide between the a and B strands of the V1/V2 domain of HIV-1 gp120 confer resistance to antibodies that neutralize CRF01-AE viruses Distribution of Naturally Occurring Norovirus Genogroups I, II, and IV in Oyster Tissues Prevalence of HIV infection, access to HIV care, and response to antiretroviral therapy among partners of HIV-infected individuals in Thailand HIV-1- specific IgA monoclonal antibodies from an HIV-1 vaccinee mediate galactosylceramide blocking and phagocytosis NATURALLY OCCURRING NANOMATRIX STRUCTURE FORMED IN NATURAL RUBBER AND ITS EFFECT ONVISCOELASTIC PROPERTIES National Survey of Pretreatment HIV-Drug Resistance in Thai HIV-1-Infected Adults

คัดลอก URL

ชื่อเรื่อง (EN):	Naturally Occurring Mutations in HIV-1 CRF01_AE Capsid Affect Viral Sensitivity to Restriction Factors
ผู้แต่ง / หัวหน้าโครงการ (EN):	Akatsuki Saito
บทคัดย่อ (EN):	TRIM5a and MxB are known as restriction factors that inhibit the early step of intracellular HIV-1 replication cycle. Both factors are believed to interact with the incoming virus core to suppress HIV-1 infection. The extreme diversity of HIV-1 is thought to be a consequence of its propensity to mutate to escape immune responses and host restriction factors. We recently determined the capsid sequences for 144 HIV-1 CRF01_AE viruses obtained in Thailand from 2005 to 2011. In this study, we further analyzed the amino acid variations among the capsid sequences of 204 HIV-1 CRF01_AE obtained in Thailand and China, including 84 of the aforementioned 144 viruses, to detect mutations permitting escape from restriction by host factors. We found a characteristic combination of E79D, V83T, and H87Q in sequences from Chinese viruses and subsequently showed that this combination conferred partial resistance to MxB. Interestingly, this combination conferred resistance to human TRIM5a as well. The H87Q mutation alone conferred resistance to MxB in the CRF01_AE background, but not in subtype B virus. In contrast, the H87Q mutation alone conferred resistance to human TRIM5a in both the CFR01_AE and subtype B backgrounds. BLAST analysis revealed the presence of the E79D, V83T, and H87Q combination in CRF01_AE viruses isolated not only in China but also in many other countries. Although the mechanistic details as well as precise role of MxB antiviral activity in infected individuals remain to be clarified, our data suggest an interaction between MxB and the HIV-1 capsid in vivo.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=518
เผยแพร่โดย (EN):	1 Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. 2 Thailand-Japan Research Collaboration Center on Emerging and Re-Emerging Infections (RCC-ERI), Osaka University, Nonthaburi,Thailand. 3 National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi, Thailand.
คำสำคัญ (EN):	TRIM5a; MxB; CRF01_AE; capsid
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science