สืบค้นงานวิจัย
Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa
Atichartpongkul S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa
ผู้แต่ง / หัวหน้าโครงการ (EN): Atichartpongkul S.
บทคัดย่อ (EN): Pseudomonas aeruginosa ohrR and ospR are gene homologs encoding oxidant sensing transcription regulators. OspR is known to regulate gpx, encoding a glutathione peroxidase, while OhrR regulates the expression of ohr that encodes an organic peroxide specific peroxiredoxin. Here, we show that ospR mediated gpx expression, like ohrR and ohr, specifically responds to organic hydroperoxides as compared to hydrogen peroxide and superoxide anion. Furthermore, the regulation of these two systems is interconnected. OspR is able to functionally complement an ohrR mutant, i.e. it regulates ohr in an oxidant dependent manner. In an ohrR mutant, in which ohr is derepressed, the induction of gpx expression by organic hydroperoxide is reduced. Likewise, in an ospR mutant, where gpx expression is constitutively high, oxidant dependent induction of ohr expression is reduced. Moreover, in vitro binding assays show that OspR binds the ohr promoter, while OhrR binds the gpx promoter, albeit with lower affinity. The binding of OhrR to the gpx promoter may not be physiologically relevant; however, OspR is shown to mediate oxidant-inducible expression at both promoters. Interestingly, the mechanism of OspR-mediated, oxidant-dependent induction at the two promoters appears to be distinct. OspR required two conserved cysteines (C24 and C134) for oxidant-dependent induction of the gpx promoter, while only C24 is essential at the ohr promoter. Overall, this study illustrates possible connection between two regulatory switches in response to oxidative stress. © 2016 Atichartpongkul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990059027&doi=10.1371%2fjournal.pone.0161982&partnerID=40&md5=08afecc90782607d6da5f23794b725b9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): tert-Butylhydroperoxide
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Atichartpongkul S.. "Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa". ไม่ระบุหน่วยงาน. ม.ป.ป.

Atichartpongkul S.. "Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa
Atichartpongkul S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Pseudomonas aeruginosa IscR-regulated ferredoxin NADP(+) reductase gene (fprB) functions in iron-sulfur cluster biogenesis and multiple stress response Pseudomonas aeruginosa nfuA: Gene regulation and its physiological roles in sustaining growth under stress and anaerobic conditions and maintaining bacterial virulence Comparison in Hydraulic Response to Water Stress Tolerance of Two Rubber Clones The OxyR-regulated phnW gene encoding 2-aminoethylphosphonate:pyruvate aminotransferase helps protect Pseudomonas aeruginosa from tert-butyl hydroperoxide Pseudomonas aeruginosa glutathione biosynthesis genes play multiple roles in stress protection, bacterial virulence and biofilm formation The FinR-regulated essential gene fprA, encoding ferredoxin NADP+ reductase: Roles in superoxide-mediated stress protection and virulence of Pseudomonas aeruginosa Physiological and comparative proteomic analyses of Thai jasmine rice and two check cultivars in response to drought stress ECOPHYSIOLOGICAL RESPONSE OF YOUNG RUBBER BUDINGS TO DROUGHT STRESS Epidemiology and risk factors of extensively drug-resistant Pseudomonas aeruginosa infections Targeting the Cell Stress Response of Plasmodium falciparum to Overcome Artemisinin Resistance
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair