สืบค้นงานวิจัย
Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion
Mathias D.K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion
ผู้แต่ง / หัวหน้าโครงการ (EN): Mathias D.K.
บทคัดย่อ (EN): Novel strategies to directly thwart malaria transmission are needed to maintain the gains achieved by current control measures. Transmission-blocking interventions (TBIs), namely vaccines and drugs targeting parasite or mosquito molecules required for vector-stage parasite development, have been recognized as promising approaches for preventing malaria transmission. However, the number of TBI targets is limited and their degree of conservation among the major vector-parasite systems causing human disease is unclear. Therefore, discovery and characterization of novel proteins involved in vector-stage parasite development of Plasmodium falciparum and Plasmodium vivax is paramount. We mined the recent Anopheles gambiae midgut lipid raft proteome for putative mosquito-derived TBI targets and characterized a secreted glycoconjugate of unknown function, AgSGU. We analyzed molecular variation in this protein among a range of anopheline mosquitoes, determined its transcriptomic and proteomic profiles, and conducted both standard and direct membrane feeding assays with P. falciparum (lab/field) and P. vivax (field) in An. gambiae and Anopheles dirus. We observed that α-AgSGU antibodies significantly reduced midgut infection intensity for both lab and field isolates of P. falciparum in An. gambiae and An. dirus. However, no transmission-reducing effects were noted when comparable concentrations of antibodies were included in P. vivax-infected blood meals. Although antibodies against AgSGU exhibit transmission-reducing activity, the high antibody titer required for achieving 80% reduction in oocyst intensity precludes its consideration as a malaria mosquito-based TBI candidate. However, our results suggest that P. falciparum and P. vivax ookinetes use a different repertoire of midgut surface glycoproteins for invasion and that α-AgSGU antibodies, as well as antibodies to other mosquito-midgut microvillar surface proteins, may prove useful as tools for interrogating Plasmodium-mosquito interactions. © 2014 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84912102674&doi=10.1016%2fj.meegid.2014.05.025&partnerID=40&md5=246a876913b5f92115230d1f7aafb01a
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Sequence Alignment
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Mathias D.K.. "Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion". ไม่ระบุหน่วยงาน. ม.ป.ป.

Mathias D.K.. "Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion
Mathias D.K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 10 Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 Artemisinin-resistant Plasmodium falciparum malaria Blood-stage parasitaemia and age determine Plasmodium falciparum and P. vivax gametocytaemia in Papua New Guinea Inhibition of merozoite invasion and transient de-sequestration by sevuparin in humans with Plasmodium falciparum malaria Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the China-Myanmar border area Sequence variation in Plasmodium falciparum merozoite surface protein-2 is associated with virulence causing severe and cerebral malaria Diversifying selection on the thrombospondin-related adhesive protein (TRAP) gene of plasmodium falciparum in Thailand Insights into the naturally acquired immune response to Plasmodium vivax malaria Microgeographically diverse Plasmodium vivax populations at the Thai-Myanmar border
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