สืบค้นงานวิจัย
Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis
Petchampai N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis
ผู้แต่ง / หัวหน้าโครงการ (EN): Petchampai N.
บทคัดย่อ (EN): Vacuolar (V)-ATPase is a proton-translocating enzyme that acidifies cellular compartments for various functions such as receptor-mediated endocytosis, intracellular trafficking and protein degradation. Previous studies in Dermacentor variabilis chronically infected with Rickettsia montanensis have identified V-ATPase as one of the tick-derived molecules transcribed in response to rickettsial infection. To examine the role of the tick V-ATPase in tick-Rickettsia interactions, a full-length 2887-bp cDNA (2532-bp open reading frame) clone corresponding to the transcript of the V0 domain subunit a of D. variabilisâ€...V-ATPase (DvVATPaseV0a) gene encoding an 843 amino acid protein with an estimated molecular weight of ∼96 kDa was isolated from D. variabilis. Amino acid sequence analysis of DvVATPaseV0a showed the highest similarity to VATPaseV0a from Ixodes scapularis. A potential N-glycosylation site and eight putative transmembrane segments were identified in the sequence. Western blot analysis of tick tissues probed with polyclonal antibody raised against recombinant DvVATPaseV0a revealed the expression of V-ATPase in the tick ovary. Transcriptional profiles of DvVATPaseV0a demonstrated a greater mRNA expression in the tick ovary, compared with the midgut and salivary glands; however, the mRNA level in each of these tick tissues remained unchanged after infection with R. montanensis for 1 h. V-ATPase inhibition bioassays resulted in a significant decrease in the ability of R. montanensis to invade tick cells in vitro, suggesting a role of V-ATPase in rickettsial infection of tick cells. Characterization of tick-derived molecules involved in rickettsial infection is essential for a thorough understanding of rickettsial transmission within tick populations and the ecology of tick-borne rickettsial diseases. © 2013 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of The Royal Entomological Society.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891735481&doi=10.1111%2fimb.12059&partnerID=40&md5=b902d8d1a4bef15066cc1a2731f2f3dc
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): United States
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง


TARR Wordcloud:
Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis
Petchampai N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector Molecular detection and characterization of noroviruses in river water in Thailand ผลงานนำเสนอประชุมวิชาการกระทรวงสาธารณสุข ปีงบประมาณ 2559 เรื่อง Rickettsia Rickettsia felis felis investigation in dog fleas investigation in dog fleas by in by in-house molecular techniques house mol Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii Molecular characterization of serine protease inhibitor isoform 3, SmSPI, from Schistosoma mansoni Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก