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Antimicrobial resistance, extended-spectrum b-Lactamase productivity, and class 1 integrons in Escherichia coli from healthy swine
Changkaew K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Antimicrobial resistance, extended-spectrum b-Lactamase productivity, and class 1 integrons in Escherichia coli from healthy swine
ผู้แต่ง / หัวหน้าโครงการ (EN): Changkaew K.
บทคัดย่อ (EN): Administration of antimicrobials to food-producing animals increases the risk of higher antimicrobial resistance in the normal intestinal flora of these animals. The present cross-sectional study was conducted to investigate antimicrobial susceptibility and extended-spectrum b-lactamase (ESBL)-producing strains and to characterize class 1 integrons in Escherichia coli in healthy swine in Thailand. All 122 of the tested isolates had drug-resistant phenotypes. High resistance was found to ampicillin (98.4% of isolates), chloramphenicol (95.9%), gentamicin (78.7%), streptomycin (77.9%), tetracycline (74.6%), and cefotaxime (72.1%). Fifty-four (44.3%) of the E. coli isolates were confirmed as ESBL-producing strains. Among them, blaCTX-M (45 isolates) and blaTEM (41 isolates) were detected. Of the blaCTX-M-positive E. coli isolates, 37 carried the blaCTX-M-1 cluster, 12 carried the blaCTX-M-9 cluster, and 5 carried both clusters. Sequence analysis revealed blaTEM-1, blaTEM-135, and blaTEM-175 in 38, 2, and 1 isolate, respectively. Eighty-seven (71%) of the 122isolates carried class 1 integrons, and eight distinct drug-resistance gene cassettes with seven different integron profiles were identified in 43 of these isolates. Gene cassettes were associated with resistance to aminoglycosides (aadA1, aadA2, aadA22, or aadA23), trimethoprim (dfrA5, dfrA12, or dfrA17), and lincosamide (linF). Genes encoding b-lactamases were not found in class 1 integrons. This study is the first to report ESBLproducing E. coli with a class 1 integron carrying the linF gene cassette in swine in Thailand. Our findings confirm that swine can be a reservoir of ESBL-producing E. coli harboring class 1 integrons, which may become a potential health risk if these integrons are transmitted to humans. Intensive analyses of animal, human, and environmental isolates are needed to control the spread of ESBL-producing E. coli strains. Copyright © International Association for Food Protection.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938388354&doi=10.4315%2f0362-028X.JFP-14-445&partnerID=40&md5=bcedee1100dd73415ad7d5672dae6441
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Trimethoprim
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Antimicrobial resistance, extended-spectrum b-Lactamase productivity, and class 1 integrons in Escherichia coli from healthy swine
Changkaew K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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