สืบค้นงานวิจัย
Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation
Kornthong N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation
ผู้แต่ง / หัวหน้าโครงการ (EN): Kornthong N.
บทคัดย่อ (EN): The central nervous system (CNS) is often intimately involved in reproduction control and is therefore a target organ for transcriptomic investigations to identify reproduction-associated genes. In this study, 454 transcriptome sequencing was performed on pooled brain and ventral nerve cord of the female mud crab (Scylla olivacea) following serotonin injection (5 μg/g BW). A total of 197,468 sequence reads was obtained with an average length of 828 bp. Approximately 38.7% of 2,183 isotigs matched with significant similarity (E value < 1e-4) to sequences within the Genbank non-redundant (nr) database, with most significant matches being to crustacean and insect sequences. Approximately 32 putative neuropeptide genes were identified from nonmatching blast sequences. In addition, we identified full-length transcripts for crustacean reproductive-related genes, namely farnesoic acid o-methyltransferase (FAMeT), estrogen sulfotransferase (ESULT) and prostaglandin F synthase (PGFS). Following serotonin injection, which would normally initiate reproductive processes, we found up-regulation of FAMeT, ESULT and PGFS expression in the female CNS and ovary. Our data here provides an invaluable new resource for understanding the molecular role of the CNS on reproduction in S. olivacea. © 2014 Kornthong et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919932470&doi=10.1371%2fjournal.pone.0115867&partnerID=40&md5=6a0567c9e484a3b1a9ffee87c37d72be
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Transcriptome
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Kornthong N.. "Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kornthong N.. "Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation
Kornthong N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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