สืบค้นงานวิจัย
Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.
Inthima P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.
ผู้แต่ง / หัวหน้าโครงการ (EN): Inthima P.
บทคัดย่อ (EN): Artemisia annua L. is a commercial source of artemisinin. Nevertheless, artemisinin content within the plant is relatively low and varies depending on genotype and environment. To broaden the genetic variability, the mutation effect of 12C-ion beam irradiation on A. annua was examined. Irradiation at 2.5 Gy had a slight lethal effect to nodal segments while a noticeable lethal effect was observed at 5 and 10 Gy. Furthermore, at higher doses (20 and 50 Gy), a severe lethal effect was observed. Mutations at the DNA level of axillary bud-derived shoots were performed by RAPD. The mutation frequency at 10 Gy was about 1.7 and 2.1 times higher than that at 2.5 and 5 Gy, respectively. After growth and artemisinin production observation of 72 irradiated mutants, around 14 and 7 % of them showed higher artemisinin content and artemisinin yield compared to the controls, respectively. The highest artemisinin content in a mutant was 1.43 % DW, which was 3.2-fold higher than the original wild type. Additionally, the highest artemisinin yield in mutants was 3.68 mg/plant, which was around 1.4-fold higher than in the wild type. Moreover, irradiated mutants exhibited antibacterial activity against S. aureus, but the wild types did not. This study presents an effective application of heavy ion beam irradiation to create variations and improve artemisinin production in A. annua. © 2014, Springer Science+Business Media Dordrecht.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84910152634&doi=10.1007%2fs11240-014-0519-z&partnerID=40&md5=a9a092d1537b130aa7c8affc2a5f63aa
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Irradiation
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Inthima P.. "Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.". ไม่ระบุหน่วยงาน. ม.ป.ป.

Inthima P.. "Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.
Inthima P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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