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Characterisation of Classical Enterotoxins, Virulence Activity, and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Thai Fermented Pork Sausages, Clinical Samples, and Healthy Carriers in Northeastern Thailand
Wanwisa Sankomkai - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Characterisation of Classical Enterotoxins, Virulence Activity, and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Thai Fermented Pork Sausages, Clinical Samples, and Healthy Carriers in Northeastern Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Wanwisa Sankomkai
บทคัดย่อ (EN): Introduction: Contamination by Staphylococcus aureus of food produced from animal sources may have diverse and multifactorial causes that depend on geographical distribution. The goal of this study was to isolate and characterise S. aureus strains from contaminated fermented pork sausage, which is a local food of northeastern Thailand. Material and methods: S. aureus strains were isolated from local pork sausage, and the presence of classical enterotoxins was determined by PCR and reversed passive latex agglutination. These results were compared with strains derived from hospitalised patients and healthy carriers. Additionally, production of extracellular enzymes and haemolysin, biofilm formation, and antibiotic susceptibility were assessed. Results: S. aureus was identified in 36 sausage isolates (60%). The strains positive for staphylococcal enterotoxin A were more frequently found in isolates from sausage and healthy carriers than in those from patients. All tested S. aureus strains were positive for DNase, lipase, proteinase, haemolysin, and biofilm formation; notably, strains isolated from food and healthy carriers displayed similar values. Most isolates were resistant to penicillin and ampicillin, while none were to methicillin. Conclusions: Thai fermented pork sausages are associated with a high risk of staphylococcal food poisoning, which may be linked to contamination caused by carriers. Dissemination of knowledge regarding best practices in sanitation and hygiene is important in local communities.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=580
เผยแพร่โดย (EN): 1 Unit of Water and Food Analysis, Division of Research, Amnatcharoen, 37000, Thailand 2 Department of Sciences and Liberal Arts, Mahidol University, Amnatcharoen Campus, Amnatcharoen, 37000, Thailand 3 Microbiology Laboratory, Division of Clinical Pathology, Amnatcharoen Hospital, Amnatcharoen, 37000, Thailand 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand
คำสำคัญ (EN): Thailand
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Characterisation of Classical Enterotoxins, Virulence Activity, and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Thai Fermented Pork Sausages, Clinical Samples, and Healthy Carriers in Northeastern Thailand
Wanwisa Sankomkai
1 Unit of Water and Food Analysis, Division of Research, Amnatcharoen, 37000, Thailand 2 Department of Sciences and Liberal Arts, Mahidol University, Amnatcharoen Campus, Amnatcharoen, 37000, Thailand 3 Microbiology Laboratory, Division of Clinical Pathology, Amnatcharoen Hospital, Amnatcharoen, 37000, Thailand 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand
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