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Candida shehatae and Saccharomyces cerevisiae work synergistically to improve ethanol fermentation from sugarcane bagasse and rice straw hydrolysate in immobilized cell bioreactor
Meethit P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Candida shehatae and Saccharomyces cerevisiae work synergistically to improve ethanol fermentation from sugarcane bagasse and rice straw hydrolysate in immobilized cell bioreactor
ผู้แต่ง / หัวหน้าโครงการ (EN): Meethit P.
บทคัดย่อ (EN): Sugarcane bagasse (SCB) and rice straw (RS), abundant lignocellulosic agro-industrial residues in South-East Asia, are potent feedstocks for bioethanol production as they contain significant amount of glucose and xylose monomers after fractionation and subsequent enzymatic hydrolysis. To simultaneously convert glucose and xylose to ethanol, it requires co-cultivation of Saccharomyces cerevisiae and Candida shehatae which are hexose and pentose-fermenting yeasts, respectively. Xylose-fermenting strain grows slower than glucose-fermenting one, therefore low efficiency of xylose-to-ethanol conversion was found. To enhance the efficiency of ethanol fermentation, the present work proposed to improve xylose assimilation by using co-immobilization of two strains in a packed bed bioreactor and to increase oxygenation of the medium by applying a recycled batch system when the recycle stream was intervened by a mixing system in a naturally aerated vessel. Initially, conversion of glucose and xylose to ethanol using pure culture was investigated. Subsequently, influence of different immobilization techniques was investigated. Cells entrapment in Ca-alginate beads provided considerably high ethanol yield over cells immobilized on delignified cellulose, and thus it was selected to use as inoculum in an immobilized cell bioreactor (ICB). The results showed that continuous ethanol production yielded 0.38 and 0.40 g/g corresponding to 74.5% and 78.4% theoretical yields from SCB and RS hydrolysate, respectively. However, recycled batch system produced significantly improved ethanol yield to 0.49 g/g and 0.50 g/g corresponding to 96.1% and 98.0% theoretical yields for SCB and RS hydrolysate, respectively. In this study, higher ethanol concentration and less unfermented sugar concentration was successfully achieved in the ICB with recycled batch system when using SCB and RS hydrolysate as the substrate. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84977576897&doi=10.1002%2felsc.201500147&partnerID=40&md5=a4509328f04d05776fd5fd5b9b7c8dd8
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Saccharomyces cerevisiae
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Candida shehatae and Saccharomyces cerevisiae work synergistically to improve ethanol fermentation from sugarcane bagasse and rice straw hydrolysate in immobilized cell bioreactor
Meethit P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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