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TLR-4 gene polymorphism in Indian Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin,37(1))
Indrasen Chauhan - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): TLR-4 gene polymorphism in Indian Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin,37(1))
ผู้แต่ง / หัวหน้าโครงการ (EN): Indrasen Chauhan
หน่วยงานสังกัดผู้แต่ง (EN): Rajasthan (India)
บทคัดย่อ (EN): TLR-4 is an important candidate gene, which affects the host disease resistance. Its role in pathogen recognition and subsequent initiation of the inflammatory and immune responses makes it a suitable candidate gene for enhancing disease resistance in dairy animals. TLR-4 gene also provides an ideal model to study the consequences of genetic variation and their relation to the function of receptor and their susceptibility to diseases. TLR-4 gene mainly recognizes the conserved lipopolysaccharide and lipoteichoic acid patterns of Gram-negative and Gram-positive bacteria, respectively. The present study was undertaken with the objectives of sequence characterization and studying the genetic variation in exon 3 of Toll-like receptor 4 (TLR-4) gene in Murrah buffalo by PCR-RFLP. Exon 3 (2265 bp) of TLR-4 gene was amplified by PCR using oligonucleotide primers designed by Primer 3 plus software and subsequently, RFLP study was carried out to identify genotypes of the animals with Bsp1286I restriction enzyme. It exhibited AA, AB and BB genotypes in exon 3 for primer 3.1, 3.5, 3.6 and 3.7. Amplicons were sequenced and compared with published Bubalus bubalis (EU386358) sequence and variation at four nucleotide positions were occurs and out of which one non synonymous SNP result into change of amino acid (Threonine to Glutamine).
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/376658
คำสำคัญ (EN): India
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TLR-4 gene polymorphism in Indian Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin,37(1))
Indrasen Chauhan
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Developing feeding module for increasing milk production in Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin (Thailand),37(1)) Hydrallantois in buffaloes (Buffalo Bulletin,37(3)) Screening for genetic disorders in Indian Murrah and Surti buffalo (Bubalus bubalis) bulls (Buffalo Bulletin (Thailand),36(1)) Aetiological and therapeutic investigations on leukoderma in Indian buffaloes (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis in Murrah buffalo (Bubalus bubalis) (Buffalo Bulletin,38(3)) Water buffalo (Bubalus bubalis) susceptibility to bovine tuberculosis is influenced by g.4002c GT t polymorphism in interleukin-10 gene (Buffalo Bulletin,38(1)) Effect of ageing on physicochemical properties and textural changes in hot boned meat from young and old Indian water buffaloes (Bubalus bubalis) (Buffalo Bulletin,37(2)) Cryopreservation of buffalo (Bubalus bubalis) semen: Current status and future prospective (Buffalo Bulletin,37(2)) Pregnancy enhancement using ovsynch protocol in Murrah buffaloes (Buffalo Bulletin,37(4)) Prevelance of fallopian tube pathologies in buffaloes (Bubalus bubalis) (Buffalo Bulletin,40(2))
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