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Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefasciatus
Uruyakorn Chansang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefasciatus
ผู้แต่ง / หัวหน้าโครงการ (EN): Uruyakorn Chansang
บทคัดย่อ (EN): Insecticide resistance poses a serious obstacle to the control of insect vectors. The microorganisms Bacillus thuringiensis subsp. israelensis (Bti) and Bacillus sphaericus (Bsph) have been used as alternative larvicides to a variety of mosquitoes such as Culex quinquefasciatus which was reported to be resistant to some groups of insecticides. National Institute of Health (NIH), Thailand has authority and function in the development of knowledge on public health. A Bti was developed in this study and tested following the technical specifications of ISO 17025:2017. C. quinquefasciatus Say 1823, a major vector of filariasis in the neighbouring countries has been monitored at the borders of Thailand. The effectiveness and appropriate long-term use of Bti, together with the potential for recovery from insecticide resistance, were tested against the standard susceptible strain of C. quinquefasciatus by a selection pressure method. Also, the commercial Bsph and temephos were tested in parallel to determine the suitable time of use. The powdered Bti was produced with a potency of 20,839.98 ITU/mg. The bioassay performed with Bti, Bsph and temephos to standard C. quinquefasciatus colonies showed 1.86-, 22.31- and 4.25-fold increases in resistance, respectively, in the 25th generation. The tests were continued without further larvicide exposure to determine the interval over which the resistance decreased. The resistance of the mosquitoes to Bti, Bsph and temephos declined to 0.99, 10.64 and 1.03 in the 31st, 68th and 41 st generations, respectively. These studies indicated that the laboratory based-Bti is effective and should be developed as a larvicidal product. C. quinquefasciatus developed resistance to Bti slowly, and the induced resistance declined rapidly compared to the resistance to Bsph and temephos.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=561
เผยแพร่โดย (EN): National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
คำสำคัญ (EN): Resistance induction.
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefasciatus
Uruyakorn Chansang
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
ไม่ระบุวันที่เผยแพร่
Monitoring of resistance in laboratory susceptible strain and field population strains of Culex quinquefasciatus to Bacillus thuringiensis var. israelensis (Bti), Bacillus sphaericus (Bsph) and temeph Efficiency, Toxicity and Persistence of Temephos (Sand Granule), Bacillus thuringiensis var. israelensis and Novaluron on Aedes aegypti L. Larvae in Sawaeng Ha district, Ang Thong province Thai Cosmetopoeia การขับเคลื่อนเศรษฐกิจสังคมไทยด้วยการสร้างสรรคุณค่าเครื่องสำอางตามเอกลักษณ์ท้องถิ่น Development of B-Soy starter for biolarvicide production for control of Culex quinquefasciatus mosquito larvae Thai Open GLAM Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin Simulations to compare efficacies of tetravalent dengue vaccines and mosquito vector control T‑SPOT®.TB Test for Latent Tuberculosis infection Diagnosis and Treatment Guidance in Thai Health‑Care Professionals Larvicidal and Repellent Activities of Crude Extracts and Essential Oils from Eupatorium odorata Against Aedes aegypti, Culex quinquefasciatus and Anopheles dirus B การตรวจจำแนกเชื้อ Bacillus cereus และ Bacillus thuringiensis ด้วยเทคนิค multiplex PCR Differentiation of Bacillus cereus and Bacillus thuringiensis by multiplex PCR assay
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