สืบค้นงานวิจัย
Distribution and genetic deversity of begomoviruses infecting tomato, pepper and cucurbits in Thailand
แสงสูรย์ เจริญวิไลศิริ - สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
ชื่อเรื่อง: Distribution and genetic deversity of begomoviruses infecting tomato, pepper and cucurbits in Thailand
ชื่อเรื่อง (EN): Distribution and genetic deversity of begomoviruses infecting tomato, pepper and cucurbits in Thailand
ผู้แต่ง / หัวหน้าโครงการ: แสงสูรย์ เจริญวิไลศิริ
หน่วยงานสังกัดผู้แต่ง:
บทคัดย่อ (EN): Begomoviruses are single-stranded DNA viruses in the family Geminiviridae that cause significant yield losses in a wide variety of economically important crops worldwide. The typical symptoms of begomovirus infection are leaf-curling, leaf yellowing, yellow veining, and stunting of plants. In Thailand, begomoviruses are constantly reported as major threats to tomato, pepper and cucurbits production fields in various parts of the country. The information regarding the distribution and genetic diversity of begomoviruses in different host plants and locations is essential for the study of virus epidemiology and for the development of strategies for effective management of begomoviruses (i.e. early detection and breeding for resistance). The aim of this project is to investigate the distribution and genetic diversity of begomoviruses in tomato, pepper and cucurbits production fields from different parts of Thailand. Plant samples showing begomovirus-liked symptoms will be collected from 10 economically important production fields in northern, northeastern, central and southern regions of Thailand (at least 100 samples/plant species). Weeds growing around production fields that show begomovirus-like symptoms will also be collected as possible natural reservoirs of begomoviruses. Begomoviruses in the symptomatic plants will be detected by enzyme-linked immunosorbent assay using in-house monoclonal antibodies to general begomoviruses and Tomato yellow leaf curl Thailand virus (TYLCTHV) and commercial monoclonal antibody to begomoviruses. Begomovirus infection in the same sample set will also be determined by PCR using universal primers for begomovirus coat protein (CP) gene and replication-associated protein (Rep) gene. The types of begomovirus genome component (monopartite vs bipartite) will be determined by PCR amplification of nuclear shuttle protein (NSP) gene. The species of begomoviruses from our survey will be identified by DNA analysis of the amplified CP, Rep and NSP gene fragments using BLAST search and phylogenetic analysis. Genetic diversity of begomoviruses will be determined by PCR amplification of the intergenic region (IR), the most variable region on DNA genome, followed by phylogenetic analysis of the amplified IR fragments. Preliminary studies on alternative host range of begomoviruses and whitefly transmissibility will also be performed using TYLCTHV and Bemisia tabaci as a model.<br />We expect that the information obtained from our study will help elucidate the current situation of begomovirus infection in tomato, pepper and cucurbits production fields in Thailand. This information can aid farmers and seed companies in selecting and breeding of begomovirus-resistance cultivars. The information can also be used as basic knowledge for further study on virus epidemiology, virus-host range and virus-vector relationships as well as for the development of tools for begomovirus screening such as the production monoclonal antibodies for begomoviruses in Thailand.</p>
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
เผยแพร่โดย: สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
คำสำคัญ (EN): whitefly transmission
เจ้าของลิขสิทธิ์: สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
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Distribution and genetic deversity of begomoviruses infecting tomato, pepper and cucurbits in Thailand
สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
2560
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