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Diagnostic potential of 36-55 kDa somatic antigens of Fasciola gigantica for bovine fasciolosis (Buffalo Bulletin,41(1))
Sabika Firasat - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Diagnostic potential of 36-55 kDa somatic antigens of Fasciola gigantica for bovine fasciolosis (Buffalo Bulletin,41(1))
ผู้แต่ง / หัวหน้าโครงการ (EN): Sabika Firasat
หน่วยงานสังกัดผู้แต่ง (EN): Islamabad (Pakistan). Faculty of Biological Sciences. Department of Zoology
บทคัดย่อ (EN): Fascioliasis causes huge economic losses in livestock industry by reducing the growth, fertility rate, meat and milk yield. In the present study, somatic antigens isolated from Fasciola gigantica were assessed for the early detection of infection by developing antibody detection enzyme immunoassay. The somatic antigens extracted from adult F. gigantica worms was separated on SDS-PAGE and immunogenicity was determined by Western blot (WB). The results showed polypeptides bands between 15 to 95 kDa, and most prominent bands were 15, 28, 36, 38, 55, 72 and 95 kDa polypeptides. The WB revealed a single polypeptide of size between 36 to 55 kDa as antigenic. The sensitivity and specificity of ELISA test established for 36 to 55 kDa somatic antigens was 95.45 percent (95 percent CI: 77.16 percent to 99.88 percent) and 87.1 percent (95 percent CI: 70.17 percent to 96.37 percent), respectively. Kappa value revealed that the strength of agreement is considered to be very 'good'. In house established ELISA was implemented in the field and 12.1 percent (31/256) large ruminants were found positive, while 4.68 percent (12/256) with postmortem examination. The fasciolosis did not show significant (P GT 0.05) association with host type, breed and age groups, while significant (P LT 0.01) association with host sexes. The result confirmed that in-house established ELISA test had good value for serodiagnosis of fasciolosis in cattle and buffaloes for large scale epidemiological studies.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/416652
คำสำคัญ (EN): Pakistan
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Sabika Firasat. "Diagnostic potential of 36-55 kDa somatic antigens of Fasciola gigantica for bovine fasciolosis (Buffalo Bulletin,41(1))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Sabika Firasat. "Diagnostic potential of 36-55 kDa somatic antigens of Fasciola gigantica for bovine fasciolosis (Buffalo Bulletin,41(1))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Diagnostic potential of 36-55 kDa somatic antigens of Fasciola gigantica for bovine fasciolosis (Buffalo Bulletin,41(1))
Sabika Firasat
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Epidemiological, clinico-diagnostic and therapeutic aspects of bovine respiratory disease in Indian buffaloes (Buffalo Bulletin,41(4)) Applications of transabdominal ultrasonography in bovine reproduction: A review (Buffalo Bulletin,41(2)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Analysis of export potential of buffalo meat in India (Buffalo Bulletin,40(4)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Constraints of buffalo farming in Kallakurichi district of Tamil Nadu, India (Buffalo Bulletin,41(3)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Expression and structural variations of toll-like receptor 4 gene in buffalo and cattle (Buffalo Bulletin,41(1))
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